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Recombinant organisms capable of fermenting cellobiose

Patent ·
OSTI ID:873150
 [1];  [1];  [1];  [1]
  1. Gainesville, FL
+ Show Author Affiliations
This invention relates to a recombinant microorganism which expresses pyruvate decarboxylase, alcohol dehydrogenase, Klebsiella phospho-.beta.-glucosidase and Klebsiella (phosphoenolpyruvate-dependent phosphotransferase system) cellobiose-utilizing Enzyme II, wherein said phospho-.beta.-glucosidase and said (phosphoenolpyruvate-dependent phosphotransferase) cellobiose-utilizing Enzyme II are heterologous to said microorganism and wherein said microorganism is capable of utilizing both hemicellulose and cellulose, including cellobiose, in the production of ethanol.
Research Organization:
University of Florida
DOE Contract Number:
FG02-96ER20222
Assignee:
Univ. of Florida Research Foundation, Inc. (Gainsville, FL)
Patent Number(s):
US 6102690
OSTI ID:
873150
Country of Publication:
United States
Language:
English

References (13)

Ethanol production by Zymomonas mobilis book December 1981
Cloning and expression of a β-glucosidase gene from Xanthomonas albilineans in Escherichia coli and Zymomonas mobilis journal January 1989
Approaches to broaden the substrate and product range of the ethanologenic bacterium Zymomonas mobilis by genetic engineering journal February 1993
Sugar transport by the bacterial phosphotransferase system. Molecular cloning and structural analysis of the Escherichia coli ptsH, ptsI, and crr genes. journal November 1987
The role of a phosphoenolpyruvate-dependent kinase system in beta-glucoside catabolism in Escherichia coli. journal March 1968
Discovery of a ptsHl operon, which includes a third gene (ptsT), in the thermophile Bacillus stearothermophilus journal June 1995
Ethanol production from cellobiose, amorphous cellulose, and crystalline cellulose by recombinant Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes from Clostridium thermocellum. journal January 1992
Isolation and molecular characterization of high-performance cellobiose-fermenting spontaneous mutants of ethanologenic Escherichia coli KO11 containing the Klebsiella oxytoca casAB operon. journal January 1997
Cloning of cellobiose phosphoenolpyruvate-dependent phosphotransferase genes: functional expression in recombinant Escherichia coli and identification of a putative binding region for disaccharides. journal January 1997
Use of the tac promoter and lacIq for the controlled expression of Zymomonas mobilis fermentative genes in Escherichia coli and Zymomonas mobilis. journal November 1992
Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli. journal January 1993
Cloning and sequencing of a cellobiose phosphotransferase system operon from Bacillus stearothermophilus XL-65-6 and functional expression in Escherichia coli. journal January 1993
Expression and stability of a .BETA.-glucosidase gene of Ruminococcus albus in Zymomonas mobilis. journal January 1989

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Related Subjects

-glucosidase
/431/435/999/
alcohol
alcohol dehydrogenase
beta
capable
cellobiose
cellobiose-utilizing
cellulose
decarboxylase
dehydrogenase
enzyme
ethanol
expresses
fermenting
hemicellulose
heterologous
ii
including
klebsiella
microorganism
organisms
organisms capable
phospho-
phosphoenolpyruvate-dependent
phosphotransferase
production
pyruvate
pyruvate decarboxylase
recombinant
recombinant organisms
relates
utilizing