skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Specimen illumination apparatus with optical cavity for dark field illumination

Patent ·
OSTI ID:872657

An illumination apparatus with a specimen slide holder, an illumination source, an optical cavity producing multiple reflection of illumination light to a specimen comprising a first and a second reflective surface arranged to achieve multiple reflections of light to a specimen is provided. The apparatus can further include additional reflective surfaces to achieve the optical cavity, a slide for mounting the specimen, a coverslip which is a reflective component of the optical cavity, one or more prisms for directing light within the optical cavity, antifading solutions for improving the viewing properties of the specimen, an array of materials for analysis, fluorescent components, curved reflective surfaces as components of the optical cavity, specimen detection apparatus, optical detection equipment, computers for analysis of optical images, a plane polarizer, fiberoptics, light transmission apertures, microscopic components, lenses for viewing the specimen, and upper and lower mirrors above and below the specimen slide as components of the optical cavity. Methods of using the apparatus are also provided.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
DOE Contract Number:
W-7405-ENG-48
Assignee:
Regents of University of California (Oakland, CA)
Patent Number(s):
US 5982534
OSTI ID:
872657
Country of Publication:
United States
Language:
English

References (5)

Light-directed, spatially addressable parallel chemical synthesis journal February 1991
Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors journal October 1992
Extensive polymorphisms observed in HIV–1 clade B protease gene using high–density oligonucleotide arrays journal July 1996
Matrix DNA Hybridization journal April 1993
Fluorescence array detector for large-field quantitative fluorescence cytometry journal July 1994