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Title: Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology

Patent ·
OSTI ID:872543
 [1]
  1. Knoxville, TN
+ Show Author Affiliations

An enhanced strain of Pseudomonas celllulosa was obtained by introducing a recombinant genetic construct comprising a heterologous cellulase gene operably connected to a promoter into ATCC 55702, mutagenizing the transformants by treatment with MNNG, and selecting a high cellulase producing transformant. The transformant, designated Pseudomonas cellulosa ATCC XXXX, exhibits enhanced levels of cellulase production relative to the untransformed Pseudomonas cellulosa strain #142 ATCC 55702.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
DOE Contract Number:
AC05-96OR22464
Assignee:
Lockheed Martin Energy Research Corp. (Oak Ridge, TN)
Patent Number(s):
US 5958740
OSTI ID:
872543
Country of Publication:
United States
Language:
English

References (2)

Cloning and expression of cellulase and xylanase genes in Lactobacillus plantarum journal August 1990
Cloning of an endoglucanase gene fromPseudomonas fluorescens var.cellulosa intoEscherichia coli andPseudomonas fluorescens journal June 1986

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Related Subjects

genetically
enhanced
cellulase
production
pseudomonas
cellulosa
recombinant
dna
technology
strain
celllulosa
obtained
introducing
genetic
construct
comprising
heterologous
operably
connected
promoter
atcc
55702
mutagenizing
transformants
treatment
mnng
selecting
producing
transformant
designated
xxxx
exhibits
levels
relative
untransformed
142
cellulase producing
enhanced cellulase
recombinant dna
operably connected
exhibits enhanced
construct comprising
/435/424/