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Method for the enzymatic production of hydrogen

Patent ·
OSTI ID:872469
 [1];  [2]
  1. Kingston, TN
  2. State College, PA
+ Show Author Affiliations

The present invention is an enzymatic method for producing hydrogen comprising the steps of: a) forming a reaction mixture within a reaction vessel comprising a substrate capable of undergoing oxidation within a catabolic reaction, such as glucose, galactose, xylose, mannose, sucrose, lactose, cellulose, xylan and starch. The reaction mixture further comprises an amount of glucose dehydrogenase in an amount sufficient to catalyze the oxidation of the substrate, an amount of hydrogenase sufficient to catalyze an electron-requiring reaction wherein a stoichiometric yield of hydrogen is produced, an amount of pH buffer in an amount sufficient to provide an environment that allows the hydrogenase and the glucose dehydrogenase to retain sufficient activity for the production of hydrogen to occur and also comprising an amount of nicotinamide adenine dinucleotide phosphate sufficient to transfer electrons from the catabolic reaction to the electron-requiring reaction; b) heating the reaction mixture at a temperature sufficient for glucose dehydrogenase and the hydrogenase to retain sufficient activity and sufficient for the production of hydrogen to occur, and heating for a period of time that continues until the hydrogen is no longer produced by the reaction mixture, wherein the catabolic reaction and the electron-requiring reactions have rates of reaction dependent upon the temperature; and c) detecting the hydrogen produced from the reaction mixture.

Research Organization:
LOCKHEED MARTIN ENERGY RES COR
DOE Contract Number:
AC05-96OR22464
Assignee:
Lockheed Martin Energy Research Corporation (Oak Ridge, TN)
Patent Number(s):
US 5942424
OSTI ID:
872469
Country of Publication:
United States
Language:
English

References (6)

Fermentation in the unicellular cyanobacterium Microcystis PCC7806 journal July 1994
Biological production of hydrogen from cellulose by natural anaerobic microflora journal January 1995
Direct conversion of cellulosic materials to hydrogen by Clostridium sp. strain no. 2 journal February 1995
Hydrogen production from pyruvate by enzymes purified from the hyperthermophilic archaeon, Pyrococcus furiosus : A key role for NADPH journal October 1994
Cloning, sequencing and expression of the gene encoding glucose dehydrogenase from the thermophilic archaeon Thermoplasma acidophilum journal February 1993
In vitro hydrogen production by glucose dehydrogenase and hydrogenase journal July 1996

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Related Subjects

/435/
activity
adenine
allows
amount
amount sufficient
buffer
capable
catabolic
catalyze
cellulose
comprises
comprising
continues
dehydrogenase
dependent
detecting
dinucleotide
electron-requiring
electrons
environment
enzymatic
enzymatic method
forming
galactose
glucose
heating
hydrogen
hydrogen comprising
hydrogen produced
hydrogenase
lactose
mannose
method
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nicotinamide
occur
oxidation
period
ph
phosphate
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producing hydrogen
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provide
rates
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reaction mixture
reaction vessel
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retain
starch
steps
stoichiometric
substrate
substrate capable
sucrose
sufficient
temperature
temperature sufficient
time
transfer
undergoing
undergoing oxidation
vessel
vessel comprising
xylan
xylose
yield