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Use of linalool synthase in genetic engineering of scent production

Patent ·
OSTI ID:872045

A purified S-linalool synthase polypeptide from Clarkia breweri is disclosed as is the recombinant polypeptide and nucleic acid sequences encoding the polypeptide. Also disclosed are antibodies immunoreactive with the purified peptide and with recombinant versions of the polypeptide. Methods of using the nucleic acid sequences, as well as methods of enhancing the smell and the flavor of plants expressing the nucleic acid sequences are also disclosed.

Research Organization:
Washington State University
DOE Contract Number:
FG06-88ER13869
Assignee:
Regents of University of Michigan (Ann Arbor, MI)
Patent Number(s):
US 5849526
OSTI ID:
872045
Country of Publication:
United States
Language:
English

References (73)

Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels journal January 1987
The ocs element: a 16 base pair palindrome essential for activity of the octopine synthase enhancer journal November 1987
A light-entrained circadian clock controls transcription of several plant genes. journal December 1988
The ocs-element is a component of the promoters of several T-DNA and plant viral genes. journal December 1989
Endogenous Rhythm in Opening and odor of Flowers of Cestrum Nocturnum journal May 1960
Osmophores of Stanhopea (Orchidaceae) journal September 1987
Biosynthesis of Monoterpenes: Partial Purification, Characterization, and Mechanism of Action of 1,8-Cineole Synthase journal February 1994
Purification and Characterization of S-Linalool Synthase, an Enzyme Involved in the Production of Floral Scent in Clarkia breweri journal February 1995
Structural Organization and Regulation of Transcription by RNA Polymerase I of Plant Nuclear Ribosomal RNA Genes book January 1994
AT-Rich Elements (ATREs) in the Promoter Regions of Nodulin and Other Higher Plant Genes: a Novel Class of Cis-Acting Regulatory Element? book January 1994
Expression of a soybean ?-conclycinin gene under the control of the Cauliflower Mosaic Virus 35S and 19S promoters in transformed petunia tissues journal January 1987
The tomato Cab-4 and Cab-5 genes encode a second type of CAB polypeptides localized in Photosystem II journal January 1987
Nucleotide sequence and chromosomal location of Cab-7, the tomato gene encoding the type II chlorophyll a/b-binding polypeptide of photosystem I journal January 1988
Activity of a chimeric promoter with the doubled CaMV 35S enhancer element in protoplast-derived cells and transgenic plants in maize journal February 1993
Structure and expression of the maize gene encoding the phosphoenolpyruvate carboxylase isozyme involved in C4 photosynthesis journal May 1989
Activity of constitutive promoters in various species from the Liliaceae journal August 1995
A new member of the CAB gene family: structure, expression and chromosomal location of Cab-8, the tomato gene encoding the Type III chlorophyll a/b-binding polypeptide of photosystem I journal March 1989
Molecular characterization and genetic mapping of DNA sequences encoding the Type I chlorophyll a/b-binding polypeptide of photosystem I in Lycopersicon esculentum (tomato) journal January 1987
Improvements in immunostaining samples embedded in methacrylate: localization of microtubules and other antigens throughout developing organs in plants of diverse taxa journal June 1992
Duplicate sequences with a similarity to expressed genes in the genome of Arabidopsis thaliana journal August 1993
Fragments of plastid DNA in the nuclear genome of tomato: prevalence, chromosomal location, and possible mechanism of integration journal March 1991
Concerted circadian oscillations in transcript levels of nineteen Lha/b (cab) genes in Lycopersicon esculentum (tomato) journal March 1993
Determination of steady-state mRNA levels of individual chlorophyll a/b binding protein genes of the tomato cab gene family journal December 1991
Three pollination morphs in Cimicifuga simplex; incipient speciation due to inferiority in competition journal January 1986
Rhythms of fragrance emission in flowers journal May 1988
Isolation and characterization of a maize chlorophyll a/b binding protein gene that produces high levels of mRNA in the dark journal February 1989
Floral volatiles fromClarkia breweri andC. concinna (Onagraceae): Recent evolution of floral scent and moth pollination journal January 1995
Higher plant terpenoids: A phytocentric overview of their ecological roles journal June 1994
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding journal May 1976
Terpene cyclase catalysis in organic solvent/minimal water media: Demonstration and optimization of (+)-α-pinene cyclase activity journal July 1986
Wound-inducible pinene cyclase from grand fir: Purification, characterization, and renaturation after SDS-PAGE journal February 1992
Characterization and mechanism of (4S)-limonene synthase, A monoterpene cyclase from the glandular trichomes of peppermint (Mentha x piperita) journal July 1992
A simple method for displaying the hydropathic character of a protein journal May 1982
Volatiles from flowers of Nicotiana sylvestris, N. otophora and Malus × domestica: headspace components and day/night changes in their relative concentrations journal January 1990
Glycosidically bound volatile components of Nicotiana sylvestris and N. Suaveolens flowers journal May 1992
Floral scents—a checklist of volatile compounds isolated by head-space techniques journal May 1993
[29] Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli book December 1986
[6] Use of T7 RNA polymerase to direct expression of cloned genes book January 1990
Pinene cyclases I and II. Two enzymes from sage (Salvia officinalis) which catalyze stereospecific cyclizations of geranyl pyrophosphate to monoterpene olefins of opposite configuration. journal January 1984
Biosynthesis of (+/-)-alpha-pinene and (-)-beta-pinene from geranyl pyrophosphate by a soluble enzyme system from sage (Salvia officinalis). journal March 1982
Purification of 4S-limonene synthase, a monoterpene cyclase from the glandular trichomes of peppermint (Mentha x piperita) and spearmint (Mentha spicata). journal April 1992
Method for radio-capillary gas chromatography employing a modified oxidation-reduction train and flow-through detector journal February 1990
6,7-epoxy-linalool and related oxygenated terpenoids from Carica papaya fruit journal May 1986
[44] Monoterpene and sesquiterpene cyclases book January 1985
Regulation of Monoterpene Biosynthesis in Conifer Defense book July 1992
Biosynthesis and catabolism of monoterpenoids journal October 1987
Circadian rhythm of volatile emission from flowers of Nicotiana sylvestris and N. suaveolens journal November 1991
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 journal August 1970
Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter journal February 1985
A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. journal February 1985
Identification of an essential upstream element in the nopaline synthase promoter by stable and transient assays journal August 1987
DNA sequences required for anaerobic expression of the maize alcohol dehydrogenase 1 gene journal October 1987
Maize sucrose synthase-1 promoter directs phloem cell-specific expression of Gus gene in transgenic tobacco plants journal June 1990
Systemic release of chemical signals by herbivore-injured corn. journal September 1992
Strcuture and transcription of the nopaline synthase gene region of T-DNA journal January 1983
Introns increase gene expression in cultured maize cells. journal December 1987
Floral Scent Production in Clarkia (Onagraceae) (I. Localization and Developmental Modulation of Monoterpene Emission and Linalool Synthase Activity) journal December 1994
Transformation of Tobacco, Tomato, Potato, and Arabidopsis thaliana Using a Binary Ti Vector System journal May 1986
Stable Transformation of Soybean Callus by DNA-Coated Gold Particles journal July 1988
Increased Gene Expression by the First Intron of Maize Shrunken-1 Locus in Grass Species journal December 1989
Isolation of Transcriptionally Regulated Root-Specific Genes from Tobacco journal July 1990
Defense Mechanisms of Conifers: Relationship of Monoterpene Cyclase Activity to Anatomical Specialization and Oleoresin Monoterpene Content journal May 1991
An octopine synthase enhancer element directs tissue-specific expression and binds ASF-1, a factor from tobacco nuclear extracts. journal October 1989
Two Regulatory Genes of the Maize Anthocyanin Pathway Are Homologous: Isolation of B Utilizing R Genomic Sequences journal December 1989
Visualizing mRNA expression in plant protoplasts: factors influencing efficient mRNA uptake and translation. journal March 1989
Evolution of floral scent in Clarkia: novel patterns of S-linalool synthase gene expression in the C. breweri flower. journal July 1996
Trends in floral scent chemistry in pollination syndromes: floral scent composition in moth-pollinated taxa journal November 1993
Biologically Active Compounds in Orchid Fragrances journal June 1969
Exploitation of Herbivore-Induced Plant Odors by Host-Seeking Parasitic Wasps journal November 1990
Characterization of cis-acting elements regulating transcription from the promoter of a constitutively active rice actin gene. journal August 1992
Bumblebee foraging and floral scent dimorphism: Bombus kirbyellus Curtis (Hymenoptera: Apidae) and Polemonium viscosum Nutt. (Polemoniaceae) journal June 1983
Formation of Flower Fragrance Compounds from Their Precursors by Enzymic Action during Flower Opening journal January 1993
Regulation of Seed-Set in Polemonium viscosum: Floral Scents, Pollination, and Resources journal June 1985