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Title: Method for loading lipid like vesicles with drugs of other chemicals

Patent ·
OSTI ID:871605
 [1]
  1. Richmond, CA
+ Show Author Affiliations

A method for accumulating drugs or other chemicals within synthetic, lipid-like vesicles by means of a pH gradient imposed on the vesicles just prior to use is described. The method is suited for accumulating molecules with basic or acid moieties which are permeable to the vesicles membranes in their uncharged form and for molecules that contain charge moieties that are hydrophobic ions and can therefore cross the vesicle membranes in their charged form. The method is advantageous over prior art methods for encapsulating biologically active materials within vesicles in that is achieves very high degrees of loading with simple procedures that are economical and require little technical expertise, furthermore kits which can be stored for prolonged periods prior to use without impairment of the capacity to achieve drug accumulation are described. A related application of the method consists of using this technology to detoxify animals that have been exposed to poisons with basic, weak acid or hydrophobic charge groups within their molecular structures.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
DOE Contract Number:
AC03-76SF00098
Assignee:
Regents of University of California (Alameda, CA)
Patent Number(s):
US 5762957
OSTI ID:
871605
Country of Publication:
United States
Language:
English

References (11)

[43] Light-induced pH gradients measured with spin-labeled amine and carboxylic acid probes: Application to Halobacterium halobium cell envelope vesicles book January 1982
Pyranine as a sensitive pH probe for liposome interiors and surfaces. pH gradients across phospholipid vesicles journal May 1978
NMR studies of pH-induced transport of carboxylic acids across phospholipid vesicle membranes journal March 1977
Membrane Transport of Sugars and Amino Acids journal June 1984
Preparation and characteristics of lipid vesicles journal December 1971
The response of fluorescent amines to pH gradients across liposome membranes journal August 1972
Uptake of antineoplastic agents into large unilamellar vesicles in response to a membrane potential journal June 1985
Techniques for encapsulating bioactive agents into liposomes journal June 1986
Light-induced proton gradients and internal volumes in chromatophores of Rhodopseudomonas sphaeroides journal November 1984
Uptake of safranine and other lipophilic cations into model membrane systems in response to a membrane potential journal January 1985
Catecholamine uptake and concentration by liposomes maintaining pH gradients journal November 1976

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