Method for loading lipid like vesicles with drugs of other chemicals

Mehlhorn, Rolf Joachim

Method for loading lipid like vesicles with drugs of other chemicals

Patent · Tue Jun 09 00:00:00 EDT 1998

OSTI ID:871605

Mehlhorn, Rolf Joachim ^[1]

Richmond, CA

A method for accumulating drugs or other chemicals within synthetic, lipid-like vesicles by means of a pH gradient imposed on the vesicles just prior to use is described. The method is suited for accumulating molecules with basic or acid moieties which are permeable to the vesicles membranes in their uncharged form and for molecules that contain charge moieties that are hydrophobic ions and can therefore cross the vesicle membranes in their charged form. The method is advantageous over prior art methods for encapsulating biologically active materials within vesicles in that is achieves very high degrees of loading with simple procedures that are economical and require little technical expertise, furthermore kits which can be stored for prolonged periods prior to use without impairment of the capacity to achieve drug accumulation are described. A related application of the method consists of using this technology to detoxify animals that have been exposed to poisons with basic, weak acid or hydrophobic charge groups within their molecular structures.

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA

DOE Contract Number:: AC03-76SF00098

Assignee:: Regents of University of California (Alameda, CA)

Patent Number(s):: US 5762957

OSTI ID:: 871605

Country of Publication:: United States

Language:: English

References (11)

The response of fluorescent amines to pH gradients across liposome membranes Deamer, David W.; Prince, Roger C.; Crofts, Antony R. Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 274, Issue 2 https://doi.org/10.1016/0005-2736(72)90180-0	journal	August 1972
Uptake of antineoplastic agents into large unilamellar vesicles in response to a membrane potential Mayer, Lawrence D.; Bally, Marcel B.; Hope, Michael J. Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 816, Issue 2 https://doi.org/10.1016/0005-2736(85)90497-3	journal	June 1985
Techniques for encapsulating bioactive agents into liposomes Mayer, Lawrence D.; Bally, Marcel B.; Hope, Michael J. Chemistry and Physics of Lipids, Vol. 40, Issue 2-4 https://doi.org/10.1016/0009-3084(86)90077-0	journal	June 1986
Pyranine as a sensitive pH probe for liposome interiors and surfaces. pH gradients across phospholipid vesicles Kano, Koji; Fendler, Janos H. Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 509, Issue 2 https://doi.org/10.1016/0005-2736(78)90048-2	journal	May 1978
Membrane Transport of Sugars and Amino Acids Reinhold, L.; Kaplan, A. Annual Review of Plant Physiology, Vol. 35, Issue 1 https://doi.org/10.1146/annurev.pp.35.060184.000401	journal	June 1984
Uptake of safranine and other lipophilic cations into model membrane systems in response to a membrane potential Bally, M. B.; Hope, M. J.; Van Echteld, C. J. A. Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 812, Issue 1 https://doi.org/10.1016/0005-2736(85)90522-X	journal	January 1985
Light-induced proton gradients and internal volumes in chromatophores of Rhodopseudomonas sphaeroides Melandri, B. Andrea; Mehlhorn, Rolf J.; Packer, Lester Archives of Biochemistry and Biophysics, Vol. 235, Issue 1 https://doi.org/10.1016/0003-9861(84)90258-3	journal	November 1984
Preparation and characteristics of lipid vesicles Miyamoto, Vernon K.; Stoeckenius, Walther The Journal of Membrane Biology, Vol. 4, Issue 1 https://doi.org/10.1007/BF02431974	journal	December 1971
[43] Light-induced pH gradients measured with spin-labeled amine and carboxylic acid probes: Application to Halobacterium halobium cell envelope vesicles Mehlhorn, R. J.; Probst, I. Biomembranes Part I: Visual Pigments and Purple Membranes II https://doi.org/10.1016/0076-6879(82)88046-4	book	January 1982
Catecholamine uptake and concentration by liposomes maintaining pH gradients Nichols, J. Wylie; Deamer, David W. Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 455, Issue 1 https://doi.org/10.1016/0005-2736(76)90169-3	journal	November 1976
NMR studies of pH-induced transport of carboxylic acids across phospholipid vesicle membranes Cramer, John A.; Prestegard, James H. Biochemical and Biophysical Research Communications, Vol. 75, Issue 2 https://doi.org/10.1016/0006-291X(77)91042-7	journal	March 1977

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Method for loading lipid like vesicles with drugs of other chemicals

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