skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Kits and methods of detection using cellulose binding domain fusion proteins

Abstract

A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

Inventors:
 [1]
  1. Karmey Yosef, IL
Publication Date:
Research Org.:
Univ. of California (United States)
OSTI Identifier:
871470
Patent Number(s):
US 5738984
Assignee:
Yissum Research Development Company of Hebrew University of Jerusalem (Jerusalem, IL)
DOE Contract Number:  
FG03-92ER20069
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
kits; methods; detection; cellulose; binding; domain; fusion; proteins; cbd; affinity; crystalline; chitin; disclosed; molecular; cloning; recombinant; production; products; comprising; protein; likewise; described; wide; range; applications; contemplated; including; drug; delivery; separations; diagnostic; techniques; molecular cloning; affinity separations; crystalline cellulose; including drug; likewise described; diagnostic techniques; binding domain; cellulose binding; wide range; products comprising; drug delivery; fusion products; recombinant production; domain fusion; fusion protein; fusion proteins; /435/436/

Citation Formats

Shoseyov, Oded. Kits and methods of detection using cellulose binding domain fusion proteins. United States: N. p., 1998. Web.
Shoseyov, Oded. Kits and methods of detection using cellulose binding domain fusion proteins. United States.
Shoseyov, Oded. 1998. "Kits and methods of detection using cellulose binding domain fusion proteins". United States. https://www.osti.gov/servlets/purl/871470.
@article{osti_871470,
title = {Kits and methods of detection using cellulose binding domain fusion proteins},
author = {Shoseyov, Oded},
abstractNote = {A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.},
doi = {},
url = {https://www.osti.gov/biblio/871470}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {4}
}

Works referenced in this record:

Nucleotide sequence and characteristics of endoglucanase gene engB from Clostridium cellulovorans
journal, July 1991


Nucleotide sequence of rat hsp60 (chaperonin, GroEL homolog) cDNA
journal, January 1990


Fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose
journal, February 1989


celA from Bacillus lautus PL236 encodes a novel cellulose-binding endo-beta-1,4-glucanase.
journal, January 1992


Efficient mapping of protein antigenic determinants.
journal, September 1986


Gene sequence and properties of Cell, a family E endoglucanase from Clostridium thermocellum
journal, February 1993


Nucleotide sequence of a gene from Caldocellum saccharolyticum encoding for exocellulase and endocellulase activity
journal, January 1989


Trends in biochemistry and enzymology of cellulose degradation
journal, November 1990


Nucleotide sequence of Clostridium cellulovorans gene homologous to cyclic-AMP dependent kinase
journal, January 1991


The 65-kilodalton antigen of Mycobacterium tuberculosis.
journal, January 1987


Immobilized endo-.beta.-glucosidase enriches flavor of wine and passion fruit juice
journal, June 1990


Homologous plant and bacterial proteins chaperone oligomeric protein assembly
journal, May 1988


Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A.
journal, April 1992


A mycobacterial 65-kD heat shock protein induces antigen-specific suppression of adjuvant arthritis, but is not itself arthritogenic.
journal, January 1990


Synovial fluid-derivedYersinia-reactive T cells responding to human 65-kDa heat-shock protein and heat-stressed antigen-presenting cells
journal, September 1991


Cloning of the mycobacterial epitope recognized by T lymphocytes in adjuvant arthritis
journal, January 1988


Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors.
journal, April 1985


Essential 170-kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase.
journal, March 1990


Structure of a Bacillus subtilis endo-β-l,4-glucanase gene
journal, January 1986


Cloning of Clostridiumcellulovoransendo-1,4-β-glucanase genes
journal, June 1990


Induction and therapy of autoimmune diabetes in the non-obese diabetic (NOD/Lt) mouse by a 65-kDa heat shock protein.
journal, February 1990


Molecular cloning of a Chinese hamster mitochondrial protein related to the “chaperonin” family of bacterial and plant proteins
journal, July 1989


Non–Hydrolytic Disruption of Cellulose Fibres by the Binding Domain of a Bacterial Cellulase
journal, November 1991