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Title: Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions

Patent ·
OSTI ID:871433
 [1];  [2];  [3]
  1. San Ramon, CA
  2. Tracy, CA
  3. Walnut Creek, CA
+ Show Author Affiliations

A method is provided for detecting nucleic acid sequence aberrations using two immobilization steps. According to the method, a nucleic acid sequence aberration is detected by detecting nucleic acid sequences having both a first nucleic acid sequence type (e.g., from a first chromosome) and a second nucleic acid sequence type (e.g., from a second chromosome), the presence of the first and the second nucleic acid sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. In the method, immobilization of a first hybridization probe is used to isolate a first set of nucleic acids in the sample which contain the first nucleic acid sequence type. Immobilization of a second hybridization probe is then used to isolate a second set of nucleic acids from within the first set of nucleic acids which contain the second nucleic acid sequence type. The second set of nucleic acids are then detected, their presence indicating the presence of a nucleic acid sequence aberration.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
DOE Contract Number:
W-7405-ENG-48
Assignee:
Regents of University of California (Oakland, CA)
Patent Number(s):
US 5731153
OSTI ID:
871433
Country of Publication:
United States
Language:
English

References (13)

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Rapid Translocation Frequency Analysis in Humans Decades after Exposure to Ionizing Radiation journal January 1992
A versatile acid-labile linker for modification of synthetic biomolecules journal January 1990
A cleavable biotinylating agent and its use in protein electroblots journal August 1992
A reagent for covalently attaching biotin to proteins via a cleavable connector arm journal October 1982
Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. journal December 1988
A novel method to map transcripts: Evidence for homology between an adenovirus mRNA and discrete multiple regions of the viral genome journal September 1977
Two-step affinity chromatography. Model systems and an example using biotin-avidin binding and a fluoridolyzable linker journal November 1991
Detection of bcr-abl fusion in chronic myelogeneous leukemia by in situ hybridization journal October 1990
Affinity purification method using a reversible biotinylating reagent for peptides synthesized by the solid-phase technique journal May 1993
Facile Preparation and some Applications of an Affinity Matrix with a Cleavable Connector arm Containing a Disulfide bond journal June 1987
Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. journal May 1986
Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots. journal July 1983

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Related Subjects

identification
random
nucleic
acid
sequence
aberrations
dual
capture
probes
hybridize
chromosome
regions
method
provided
detecting
immobilization
steps
according
aberration
detected
sequences
type
presence
indicating
hybridization
probe
isolate
set
acids
sample
contain
sequence aberration
detecting nucleic
sequence aberrations
sequence type
acid sequence
nucleic acid
acid sequences
nucleic acids
hybridization probe
sequence indicating
immobilization steps
/435/999/