Method for increasing thermostability in cellulase ennzymes

Patent · Mon Jan 26 23:00:00 EST 1998

OSTI ID:871345

Adney, William S ^[1]; Thomas, Steven R ^[2]; Baker, John O ^[1]; Himmel, Michael E ^[3]; Chou, Yat-Chen ^[4]

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1. Golden, CO
2. Denver, CO
3. Littleton, CO
4. Wheat Ridge, CO

The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product.

• Research Organization: Midwest Research Institute
• DOE Contract Number: AC36-83CH10093
• Assignee: Midwest Research Institute (Kansas City, MO)
• Patent Number(s): US 5712142
• OSTI ID: 871345
• Country of Publication: United States
• Language: English

Related Subjects

/435/999/
acidothermus
acidothermus cellulolyticus
binding
binding domain
catalytic
catalytic domain
cellulase
cellulolyticus
cellulolyticus e1
cellulose
cellulose binding
cleavage
cloned
comprising
demonstrates
domain
e1
e1 endoglucanase
encoding
encoding acidothermus
endoglucanase
endoglucanase enzyme
enhanced
ennzymes
enzyme
expressed
expression
genetic
increasing
linker
method
methods
modified
modified e1
pastoris
peptide
pichia
produced
producing
product
proteolytic
remove
size
thermostability
truncation