High density array fabrication and readout method for a fiber optic biosensor

Patent · Wed Jan 01 04:00:00 EST 1997

OSTI ID:871246

Pinkel, Daniel ^[1]; Gray, Joe ^[2]

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1. Walnut Creek, CA
2. San Francisco, CA

The invention relates to the fabrication and use of biosensors comprising a plurality of optical fibers each fiber having attached to its "sensor end" biological "binding partners" (molecules that specifically bind other molecules to form a binding complex such as antibody-antigen, lectin-carbohydrate, nucleic acid-nucleic acid, biotin-avidin, etc.). The biosensor preferably bears two or more different species of biological binding partner. The sensor is fabricated by providing a plurality of groups of optical fibers. Each group is treated as a batch to attach a different species of biological binding partner to the sensor ends of the fibers comprising that bundle. Each fiber, or group of fibers within a bundle, may be uniquely identified so that the fibers, or group of fibers, when later combined in an array of different fibers, can be discretely addressed. Fibers or groups of fibers are then selected and discretely separated from different bundles. The discretely separated fibers are then combined at their sensor ends to produce a high density sensor array of fibers capable of assaying simultaneously the binding of components of a test sample to the various binding partners on the different fibers of the sensor array. The transmission ends of the optical fibers are then discretely addressed to detectors--such as a multiplicity of optical sensors. An optical signal, produced by binding of the binding partner to its substrate to form a binding complex, is conducted through the optical fiber or group of fibers to a detector for each discrete test. By examining the addressed transmission ends of fibers, or groups of fibers, the addressed transmission ends can transmit unique patterns assisting in rapid sample identification by the sensor.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA
• DOE Contract Number: AC03-76SF00098
• Assignee: Regents of University of California (Oakland, CA)
• Patent Number(s): US 5690894
• OSTI ID: 871246
• Country of Publication: United States
• Language: English

Related Subjects

/422/65/250/435/
acid
acid-nucleic
addressed
antibody-antigen
array
array fabrication
assaying
assisting
attach
attached
batch
bears
bind
binding
binding partner
binding partners
biological
biosensor
biosensors
biosensors comprising
biotin-avidin
bundle
bundles
capable
combined
complex
components
comprising
conducted
density
density array
detector
detectors--such
discrete
discretely
etc
examining
fabricated
fabrication
fiber
fiber optic
fibers
form
identification
identified
lectin-carbohydrate
method
molecules
multiplicity
nucleic
nucleic acid
optic
optic biosensor
optical
optical fiber
optical fibers
optical sensor
optical sensors
optical signal
partner
partners
patterns
plurality
preferably
produce
produced
providing
rapid
readout
readout method
relates
sample
selected
sensor
sensor array
sensors
separated
signal
simultaneously
species
specifically
specifically bind
substrate
transmission
transmit
treated
unique
uniquely
various