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Title: Ethanol production in Gram-positive microbes

Patent ·
OSTI ID:870245

The subject invention concerns the transformation of Gram-positive bacteria with heterologous genes which confer upon these microbes the ability to produce ethanol as a fermentation product. Specifically exemplified is the transformation of bacteria with genes, obtainable from Zymomonas mobilis, which encode pyruvate decarboxylase and alcohol dehydrogenase.

Research Organization:
Univ. of Florida, Gainesville, FL (United States)
DOE Contract Number:
FG05-86ER13574
Assignee:
University of Florida (Gainesville, FL)
Patent Number(s):
US 5482846
OSTI ID:
870245
Country of Publication:
United States
Language:
English

References (12)

Production of ethanol byClostridium thermosaccharolyticum: II. A quantitative model describing product distributions journal December 1989
Cloning and expression of the structural gene for pyruvate decarboxylase of Zymomonas mobilis in Escherichia coli journal April 1986
Ethanol tolerance and carbohydrate metabolism in lactobacilli journal July 1992
Production of ethanol byClostridium thermosaccharolyticum: I. Effect of cell recycle and environmental parameters journal December 1989
Nucleotide sequence of the pyruvate decarboxylase gene from Zymomonas mobilis journal January 1987
Ethanol production by recombinantescherichia coli carrying genes from zymomonas mobilis journal March 1991
Production of Ethanol from d-Xylose by Using d-Xylose Isomerase and Yeasts journal January 1981
Expression of a xylanase gene of Bacillus pumilus in Escherichia coli and Bacillus subtilis journal August 1985
Genetic engineering of ethanol production in Escherichia coli. journal January 1987
Expression of theZymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes inBacillus journal May 1994
Genetic engineering of soft-rot bacteria for ethanol production from lignocellulose journal May 1993
Pyruvate decarboxylase from Zymomonas mobilis. Isolation and partial characterization journal November 1986