DNA purification by triplex-affinity capture and affinity capture electrophoresis

Cantor, Charles R; Ito, Takashi; Smith, Cassandra L

Title: DNA purification by triplex-affinity capture and affinity capture electrophoresis

Patent · Mon Jan 01 00:00:00 EST 1996

OSTI ID:870243

Cantor, Charles R ^[1]; Ito, Takashi ^[2]; Smith, Cassandra L ^[1]

Boston, MA
Chiba, JP

The invention provides a method for purifying or isolating double stranded DNA intact using triple helix formation. The method includes the steps of complexing an oligonucleotide and double stranded DNA to generate a triple helix and immobilization of the triple helix on a solid phase by means of a molecular recognition system such as avidin/biotin. The purified DNA is then recovered intact by treating the solid phase with a reagent that breaks the bonds between the oligonucleotide and the intact double stranded DNA while not affecting the Watson-Crick base pairs of the double helix. The present invention also provides a method for purifying or isolating double stranded DNA intact by complexing the double stranded DNA with a specific binding partner and recovering the complex during electrophoresis by immobilizing it on a solid phase trap imbedded in an electrophoretic gel.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

DOE Contract Number:: AC03-76SF00098

Assignee:: Regents of University of California (Oakland, CA)

Patent Number(s):: US 5482836

OSTI ID:: 870243

Country of Publication:: United States

Language:: English

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dna
purification
triplex-affinity
capture
affinity
electrophoresis
provides
method
purifying
isolating
double
stranded
intact
triple
helix
formation
steps
complexing
oligonucleotide
generate
immobilization
solid
phase
means
molecular
recognition
avidin
biotin
purified
recovered
treating
reagent
breaks
bonds
affecting
watson-crick
base
pairs
specific
binding
partner
recovering
complex
immobilizing
trap
imbedded
electrophoretic
triple helix
stranded dna
binding partner
solid phase
double stranded
affinity capture
double helix
base pairs
base pair
specific binding
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Title: DNA purification by triplex-affinity capture and affinity capture electrophoresis

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References (30)

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