Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Multiple capillary biochemical analyzer

Patent ·
OSTI ID:870013
 [1];  [1]
  1. Edmonton, CA
+ Show Author Affiliations

A multiple capillary analyzer allows detection of light from multiple capillaries with a reduced number of interfaces through which light must pass in detecting light emitted from a sample being analyzed, using a modified sheath flow cuvette. A linear or rectangular array of capillaries is introduced into a rectangular flow chamber. Sheath fluid draws individual sample streams through the cuvette. The capillaries are closely and evenly spaced and held by a transparent retainer in a fixed position in relation to an optical detection system. Collimated sample excitation radiation is applied simultaneously across the ends of the capillaries in the retainer. Light emitted from the excited sample is detected by the optical detection system. The retainer is provided by a transparent chamber having inward slanting end walls. The capillaries are wedged into the chamber. One sideways dimension of the chamber is equal to the diameter of the capillaries and one end to end dimension varies from, at the top of the chamber, slightly greater than the sum of the diameters of the capillaries to, at the bottom of the chamber, slightly smaller than the sum of the diameters of the capillaries. The optical system utilizes optic fibres to deliver light to individual photodetectors, one for each capillary tube. A filter or wavelength division demultiplexer may be used for isolating fluorescence at particular bands.

Research Organization:
University of Alberta
DOE Contract Number:
FG02-91ER61123
Assignee:
Governors of University of Alberta (Edmonton, CA)
Patent Number(s):
US 5439578
OSTI ID:
870013
Country of Publication:
United States
Language:
English

References (14)

Two-label peak-height encoded DNA sequencing by capillary gel electrophoresis: three examples journal January 1992
Formamide modified polyacrylamide gels for DNA sequencing by capillary gel electrophoresis journal January 1992
Separation of DNA sequencing fragments at 53 bases/minute by capillary gel electrophoresis journal September 1992
Multiple-sheathflow capillary array DNA analyser journal February 1993
Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence journal October 1988
Buchbesprechungen journal January 1990
Multiple Sheath-Flow Gel Capillary-Array Electrophoresis for Multicolor Fluorescent DNA Detection journal April 1994
Capillary array electrophoresis using laser-excited confocal fluorescence detection journal April 1992
Photodestruction of fluorophores and optimum conditions for trace DNA detection by automated DNA sequencer journal January 1992
Three DNA sequencing methods using capillary gel electrophoresis and laser-induced fluorescence journal December 1991
Interaction of capillary zone electrophoresis with a sheath flow cuvette detector journal March 1990
Capillary gel electrophoresis for DNA sequencing journal September 1990
Optimization of Parameters in a DNA Sequenator Using Fluorescence Detection journal July 1988
High-speed separations of DNA sequencing reactions by capillary electrophoresis journal May 1990

Similar Records

Multiple capillary biochemical analyzer
Patent · Tue Aug 08 00:00:00 EDT 1995 · OSTI ID:100979
Multiple capillary biochemical analyzer with barrier member
Patent · Sun Dec 31 23:00:00 EST 1995 · OSTI ID:870647
Multiple capillary biochemical analyzer with barrier member
Patent · Tue Oct 22 00:00:00 EDT 1996 · OSTI ID:392644

Related Subjects

/204/356/
allows
allows detection
analyzed
analyzer
applied
array
bands
biochemical
biochemical analyzer
bottom
capillaries
capillary
capillary biochemical
capillary tube
chamber
chemical analyzer
closely
collimated
cuvette
deliver
demultiplexer
detected
detecting
detecting light
detection
diameter
diameters
dimension
division
draws
emitted
equal
excitation
excitation radiation
excited
fibres
filter
fixed
fixed position
flow
flow chamber
fluid
fluorescence
held
individual
individual sample
interfaces
introduced
inward
isolating
light
light emitted
linear
modified
multiple
multiple capillaries
multiple capillary
optic
optical
optical detection
particular
pass
photodetectors
position
provided
radiation
rectangular
rectangular array
rectangular flow
reduced
relation
retainer
sample
sample stream
sample streams
sheath
sheath flow
sheath fluid
sideways
simultaneously
slanting
slightly
spaced
streams
top
transparent
tube
utilizes
varies
walls
wavelength
wavelength division
wedged