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Title: Multiple capillary biochemical analyzer

Patent ·
OSTI ID:870013
 [1];  [1]
  1. Edmonton, CA
+ Show Author Affiliations

A multiple capillary analyzer allows detection of light from multiple capillaries with a reduced number of interfaces through which light must pass in detecting light emitted from a sample being analyzed, using a modified sheath flow cuvette. A linear or rectangular array of capillaries is introduced into a rectangular flow chamber. Sheath fluid draws individual sample streams through the cuvette. The capillaries are closely and evenly spaced and held by a transparent retainer in a fixed position in relation to an optical detection system. Collimated sample excitation radiation is applied simultaneously across the ends of the capillaries in the retainer. Light emitted from the excited sample is detected by the optical detection system. The retainer is provided by a transparent chamber having inward slanting end walls. The capillaries are wedged into the chamber. One sideways dimension of the chamber is equal to the diameter of the capillaries and one end to end dimension varies from, at the top of the chamber, slightly greater than the sum of the diameters of the capillaries to, at the bottom of the chamber, slightly smaller than the sum of the diameters of the capillaries. The optical system utilizes optic fibres to deliver light to individual photodetectors, one for each capillary tube. A filter or wavelength division demultiplexer may be used for isolating fluorescence at particular bands.

Research Organization:
University of Alberta
DOE Contract Number:
FG02-91ER61123
Assignee:
Governors of University of Alberta (Edmonton, CA)
Patent Number(s):
US 5439578
OSTI ID:
870013
Country of Publication:
United States
Language:
English

References (14)

Optimization of Parameters in a DNA Sequenator Using Fluorescence Detection journal July 1988
Multiple-sheathflow capillary array DNA analyser journal February 1993
Capillary gel electrophoresis for DNA sequencing journal September 1990
Two-label peak-height encoded DNA sequencing by capillary gel electrophoresis: three examples journal January 1992
Interaction of capillary zone electrophoresis with a sheath flow cuvette detector journal March 1990
Buchbesprechungen journal January 1990
Three DNA sequencing methods using capillary gel electrophoresis and laser-induced fluorescence journal December 1991
Separation of DNA sequencing fragments at 53 bases/minute by capillary gel electrophoresis journal September 1992
High-speed separations of DNA sequencing reactions by capillary electrophoresis journal May 1990
Capillary array electrophoresis using laser-excited confocal fluorescence detection journal April 1992
Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence journal October 1988
Multiple Sheath-Flow Gel Capillary-Array Electrophoresis for Multicolor Fluorescent DNA Detection journal April 1994
Formamide modified polyacrylamide gels for DNA sequencing by capillary gel electrophoresis journal January 1992
Photodestruction of fluorophores and optimum conditions for trace DNA detection by automated DNA sequencer journal January 1992

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Related Subjects

multiple
capillary
biochemical
analyzer
allows
detection
light
capillaries
reduced
interfaces
pass
detecting
emitted
sample
analyzed
modified
sheath
flow
cuvette
linear
rectangular
array
introduced
chamber
fluid
draws
individual
streams
closely
spaced
held
transparent
retainer
fixed
position
relation
optical
collimated
excitation
radiation
applied
simultaneously
excited
detected
provided
inward
slanting
walls
wedged
sideways
dimension
equal
diameter
varies
top
slightly
diameters
bottom
utilizes
optic
fibres
deliver
photodetectors
tube
filter
wavelength
division
demultiplexer
isolating
fluorescence
particular
bands
sample stream
optical detection
excitation radiation
sheath flow
multiple capillaries
capillary tube
light emitted
fixed position
flow chamber
sheath fluid
rectangular array
wavelength division
capillary biochemical
biochemical analyzer
sample streams
multiple capillary
detecting light
chemical analyzer
individual sample
rectangular flow
allows detection
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