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Phase-sensitive flow cytometer

Patent ·
OSTI ID:869071
 [1]
  1. Los Alamos, NM
+ Show Author Affiliations
A phase-sensitive flow cytometer (FCM) provides additional FCM capability to use the fluorescence lifetime of one or more fluorochromes bound to single cells to provide additional information regarding the cells. The resulting fluorescence emission can be resolved into individual fluorescence signals if two fluorochromes are present or can be converted directly to a decay lifetime from a single fluorochrome. The excitation light for the fluorochromes is modulated to produce an amplitude modulated fluorescence pulse as the fluorochrome is excited in the FCM. The modulation signal also forms a reference signal that is phase-shifted a selected amount for subsequent mixing with the output modulated fluorescence intensity signal in phase-sensitive detection circuitry. The output from the phase-sensitive circuitry is then an individual resolved fluorochrome signal or a single fluorochrome decay lifetime, depending on the applied phase shifts.
Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM
DOE Contract Number:
W-7405-ENG-36
Assignee:
United States of America as represented by United States (Washington, DC)
Patent Number(s):
US 5270548
OSTI ID:
869071
Country of Publication:
United States
Language:
English

References (4)

Phase-Resolved Fluorescence Spectroscopy journal November 1984
Two-color immunofluorescence using a fluorescence-activated cell sorter. journal July 1977
Improved multilaser/multiparameter flow cytometer for analysis and sorting of cells and particles journal November 1991
The Measurement and Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry journal January 1984

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