Phase-sensitive flow cytometer
- Los Alamos, NM
A phase-sensitive flow cytometer (FCM) provides additional FCM capability to use the fluorescence lifetime of one or more fluorochromes bound to single cells to provide additional information regarding the cells. The resulting fluorescence emission can be resolved into individual fluorescence signals if two fluorochromes are present or can be converted directly to a decay lifetime from a single fluorochrome. The excitation light for the fluorochromes is modulated to produce an amplitude modulated fluorescence pulse as the fluorochrome is excited in the FCM. The modulation signal also forms a reference signal that is phase-shifted a selected amount for subsequent mixing with the output modulated fluorescence intensity signal in phase-sensitive detection circuitry. The output from the phase-sensitive circuitry is then an individual resolved fluorochrome signal or a single fluorochrome decay lifetime, depending on the applied phase shifts.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- DOE Contract Number:
- W-7405-ENG-36
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Number(s):
- US 5270548
- OSTI ID:
- 869071
- Country of Publication:
- United States
- Language:
- English
Phase-Resolved Fluorescence Spectroscopy
|
journal | November 1984 |
Two-color immunofluorescence using a fluorescence-activated cell sorter.
|
journal | July 1977 |
Improved multilaser/multiparameter flow cytometer for analysis and sorting of cells and particles
|
journal | November 1991 |
The Measurement and Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry
|
journal | January 1984 |
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Phase-sensitive flow cytometer
Flow cytometer for resolving signals from heterogeneous fluorescence emissions and quantifying lifetime in fluorochrome-labeled cells/particles by phase-sensitive detection
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flow
cytometer
fcm
provides
additional
capability
fluorescence
lifetime
fluorochromes
bound
single
cells
provide
information
regarding
resulting
emission
resolved
individual
signals
converted
directly
decay
fluorochrome
excitation
light
modulated
produce
amplitude
pulse
excited
modulation
signal
forms
reference
phase-shifted
selected
amount
subsequent
mixing
output
intensity
detection
circuitry
depending
applied
phase
shifts
provides additional
amplitude modulated
phase-sensitive detection
sensitive detection
modulation signal
provide additional
phase shift
reference signal
excitation light
flow cytometer
fluorescence emission
fluorescence intensity
detection circuitry
phase shifts
single cell
detection circuit
information regarding
fluorescence lifetime
selected amount
phase-sensitive flow
converted directly
additional information
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