Means and method of detection in chemical separation procedures

Patent · Fri Jan 01 00:00:00 EST 1993

OSTI ID:868691

Yeung, Edward S ^[1]; Koutny, Lance B ^[1]; Hogan, Barry L ^[1]; Cheung, Chan K ^[1]; Ma, Yinfa ^[1]

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1. Ames, IA

A means and method for indirect detection of constituent components of a mixture separated in a chemical separation process. Fluorescing ions are distributed across the area in which separation of the mixture will occur to provide a generally uniform background fluorescence intensity. For example, the mixture is comprised of one or more charged analytes which displace fluorescing ions where its constituent components separate to. Fluorescing ions of the same charge as the charged analyte components cause a displacement. The displacement results in the location of the separated components having a reduced fluorescence intensity to the remainder of the background. Detection of the lower fluorescence intensity areas can be visually, by photographic means and methods, or by automated laser scanning.

• Research Organization: Ames Laboratory (AMES), Ames, IA; Iowa State Univ., Ames, IA (United States)
• DOE Contract Number: W-7405-ENG-82
• Assignee: Iowa State University Research Foundation, Inc. (Ames, IA)
• Patent Number(s): US 5192407
• Application Number: 07/472,315
• OSTI ID: 868691
• Country of Publication: United States
• Language: English

Related Subjects

means
method
detection
chemical
separation
procedures
indirect
constituent
components
mixture
separated
process
fluorescing
distributed
occur
provide
uniform
background
fluorescence
intensity
example
comprised
charged
analytes
displace
separate
charge
analyte
displacement
results
location
reduced
remainder
visually
photographic
methods
automated
laser
scanning
direct detection
separation process
fluorescence intensity
separated components
chemical separation
laser scanning
constituent components
mixture separated
laser scan
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