Enzyme electrochemical sensor electrode and method of making it

Patent · 01 January 1992

OSTI ID:868138

Rishpon, Judith ^[1]; Zawodzinski, Thomas A ^[1]; Gottesfeld, Shimshon ^[1]

---

1. Los Alamos, NM

An electrochemical sensor electrode is formed from an electronic conductor coated with a casting solution containing a perfluorosulfonic acid ionomer and a selected enzyme. The selected enzyme catalyzes a reaction between a predetermined substance in a solution and oxygen to form an electrochemically active compound that is detected at the electronic conductor. The resulting perfluorosulfonic acid polymer provides a stable matrix for the enzyme for long lived enzyme activity, wherein only thin coatings are required on the metal conductor. The polymer also advantageously repels interfering substances from contacting the enzyme and contains quantities of oxygen to maintain a sensing capability during conditions of oxygen depletion in the sample. In one particular embodiment, glucose oxidase is mixed with the perfluorosulfonic acid ionomer to form an electrode for glucose detection.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM
• DOE Contract Number: W-7405-ENG-36
• Assignee: United States of America as represented by Department of Energy (Washington, DC)
• Patent Number(s): US 5082550
• OSTI ID: 868138
• Country of Publication: United States
• Language: English

Related Subjects

/204/435/
acid
acid ionomer
acid polymer
active
active compound
activity
advantageously
capability
casting
casting solution
catalyzes
chemical sensor
chemically active
coated
coatings
compound
conditions
conductor
contacting
containing
contains
depletion
detected
detection
electrochemical
electrochemical sensor
electrochemically
electrochemically active
electrode
electronic
electronic conductor
embodiment
enzyme
enzyme activity
enzyme catalyzes
form
formed
glucose
interfering
ionomer
lived
maintain
matrix
metal
metal conduct
metal conductor
method
mixed
oxidase
oxygen
particular
particular embodiment
perfluorosulfonic
perfluorosulfonic acid
polymer
polymer provides
predetermined
provides
quantities
reaction
repels
required
resulting
sample
selected
selected enzyme
sensing
sensor
sensor electrode
solution
solution containing
stable
substance
substances
sulfonic acid