Micro-polarimeter for high performance liquid chromatography

Patent · Tue Jan 01 00:00:00 EST 1985

OSTI ID:865329

Yeung, Edward E ^[1]; Steenhoek, Larry E ^[2]; Woodruff, Steven D ^[3]; Kuo, Jeng-Chung ^[4]

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1. Ames, IA
2. Wilmington, DE
3. Morgantown, WV
4. Skokie, IL

A micro-polarimeter interfaced with a system for high performance liquid chromatography, for quantitatively analyzing micro and trace amounts of optically active organic molecules, particularly carbohydrates. A flow cell with a narrow bore is connected to a high performance liquid chromatography system. Thin, low birefringence cell windows cover opposite ends of the bore. A focused and polarized laser beam is directed along the longitudinal axis of the bore as an eluent containing the organic molecules is pumped through the cell. The beam is modulated by air gap Faraday rotators for phase sensitive detection to enhance the signal to noise ratio. An analyzer records the beams's direction of polarization after it passes through the cell. Calibration of the liquid chromatography system allows determination of the quantity of organic molecules present from a determination of the degree to which the polarized beam is rotated when it passes through the eluent.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• DOE Contract Number: W-7405-ENG-48
• Assignee: Iowa State University Research Foundation, Inc. (Ames, IA)
• Patent Number(s): US 4498774
• OSTI ID: 865329
• Country of Publication: United States
• Language: English

Related Subjects

micro-polarimeter
performance
liquid
chromatography
interfaced
quantitatively
analyzing
micro
trace
amounts
optically
active
organic
molecules
particularly
carbohydrates
flow
cell
narrow
bore
connected
birefringence
windows
cover
opposite
focused
polarized
laser
beam
directed
longitudinal
axis
eluent
containing
pumped
modulated
air
gap
faraday
rotators
phase
sensitive
detection
enhance
signal
noise
ratio
analyzer
records
beams
direction
polarization
passes
calibration
allows
determination
quantity
degree
rotated
organic molecules
performance liquid
liquid chromatograph
sensitive detection
narrow bore
air gap
laser beam
longitudinal axis
flow cell
liquid chromatography
optically active
polarized beam
polarized laser
noise ratio
quantitatively analyzing
trace amounts
trace amount
phase sensitive
organic molecule
analyzing micro
active organic
/356/422/436/