NMR methods for in-situ biofilm metabolism studies: spatial and temporal resolved measurements

Journal Article · Tue Nov 01 04:00:00 EST 2005 · Water Science and Technology

DOI:https://doi.org/10.1016/j.mimet.2005.04.017· OSTI ID:860407

Majors, Paul D; Mclean, Jeffrey S; Fredrickson, Jim K; Wind, Robert A

We are developing nuclear magnetic resonance (NMR) microscopy, spectroscopy and combined NMR/optical techniques to the study of biofilms. Objectives include: time and depth-resolved metabolite concentrations with isotropic spatial resolution on the order of 10 microns, metabolic pathways and flux rates, mass transport and ultimately their correlation with gene expression by optical microscopy in biofilms. These methods are being developed with Shewanella oneidensis MR-1 as a model system, but are equally applicable to other biofilm systems of interest. Thus, spatially resolved NMR of biofilms is expected to contribute significantly to the understanding of adherent cell metabolism.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 860407
• Report Number(s): PNNL-SA-43300; 10895
• Journal Information: Water Science and Technology, Journal Name: Water Science and Technology Journal Issue: 7 Vol. 52; ISSN 0273-1223; ISSN WSTED4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL PATHWAYS
GENES
METABOLISM
METABOLITES
MICROSCOPY
NMR
NUCLEAR MAGNETIC RESONANCE
OPTICAL MICROSCOPY
SPATIAL RESOLUTION
SPECTROSCOPY
Shewanella
TRANSPORT
biofilm
environmental molecular sciences laboratory
metabolite
microscopy