Ultra-Sensitive, High Throughput and Quantitative Proteomics Measurements

Journal Article · Tue Feb 01 04:00:00 EST 2005 · International Journal of Mass Spectrometry, 240(3):195-212

DOI:https://doi.org/10.1016/j.ijms.2004.09.024· OSTI ID:859963

Jacobs, Jon M.; Monroe, Matthew E.; Qian, Weijun; Shen, Yufeng; Anderson, Gordon A.; Smith, Richard D.

We describe the broad basis and application of an approach for very high throughput, ultra-sensitive, and quantitative proteomic measurements based upon the use of ultra-high performance separations and mass spectrometry. An overview of the accurate mass and time (AMT) tag approach and a description of the incorporated data analysis pipeline necessary for efficient proteomic studies are presented. Adjunct technologies, including stable-isotope labeling methodologies and improvements in the utilization of LC-MS peak intensity information for quantitative purposes are discussed. Related areas include the use of automated sample handling for improving analysis reproducibility, methods for using information from the separation for more confident peptide peak identification, and the utilization of smaller diameter capillary columns having lower volumetric flow rates to increase electrospray ionization efficiency and allow for more predictable and quantitative results. The developments are illustrated in the context of studies of complex biological systems.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 859963
• Report Number(s): PNNL-SA-42919; 400412000
• Journal Information: International Journal of Mass Spectrometry, 240(3):195-212, Journal Name: International Journal of Mass Spectrometry, 240(3):195-212 Journal Issue: 3 Vol. 240
• Country of Publication: United States
• Language: English