Deletion of a coordinate regulator of type 2 cytokine expression in mice

Journal Article · Mon Jul 30 04:00:00 EDT 2001 · Nature Immunology

DOI:https://doi.org/10.1038/ni0901-842· OSTI ID:836370

Mohrs, Markus ^[1]; Blankespoor, Catherine M; Wang, Zhi-En; Loots, Gaby G; Hadeiba, Husein; Shinkai, Kanade; Rubin, Edward M; Locksley, Richard M

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1. LBNL Library

Mechanisms underlying the differentiation of stable T helper subsets will be important in understanding how discrete types of immunity develop in response to different pathogens. An evolutionarily conserved {approx}400 base pair non-coding sequence in the IL-4/IL-13 intergenic region, designated CNS-1, was deleted in mice. The capacity to develop Th2 cells was compromised in vitro and in vivo in the absence of CNS-1. Despite the profound effect in T cells, mast cells from CNS-1-deleted mice maintained their capacity to produce IL-4. A T cell-specific element critical for optimal expression of type 2 cytokines may represent evolution of a regulatory sequence exploited by adaptive immunity.

• Research Organization: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
• Sponsoring Organization: USDOE Director. Office of Science. Office of Biological and Environmental Research. Life Sciences Division; National Institutes of Health Grants HL56385 and AI30663 and HL66671 (US)
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 836370
• Report Number(s): LBNL--48789
• Journal Information: Nature Immunology, Journal Name: Nature Immunology Journal Issue: 9 Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CAPACITY
IMMUNITY
IN VITRO
IN VIVO
LYMPHOKINES
MAST CELLS
MICE
PATHOGENS