Analysis of Ig V{sub H} region genes encoding IgE antibodies in splenic B lymphocytes of a patient with asthma
Journal Article
·
· Journal of Immunology
OSTI ID:83484
- Southampton Univ. Hospitals (United Kingdom); and others
An atopic patient with hypersensitivity against house dust mite died as a result of an asthmatic attack. A portion of the spleen was obtained and was used to analyze the spectrum of Ig heavy chain V regions involved in encoding IgE Abs. A nested PCR technique generated 14 cloned V{sub H} sequences that had distinct CDR3 regions; 5 of 14 were derived from the minor V{sub H}5 family, and the remainder derived from the larger families, V{sub H}3 (6 of 14) and V{sub H}4 (3 of 14). One of the V{sub H}3-derived sequences was present as a repeated sequence in three clones. A control PCR with the same V{sub H} primers in combination with J{sub H} primers yielded only 1 of 13 sequences from V{sub H}5, indicating preferential V{sub H}5 usage only for IgE. Analysis of V{sub H}5-C{epsilon} sequences revealed usage of a single gene, DP73, with extensive mutations and several {open_quotes}hot spots{close_quotes} containing common replacement amino acids. However, there was no concentration of replacement mutations in the CDRs, which conventionally would indicate a role for Ag selection. The V{sub H}3 and V{sub H}4 genes in combination with C{epsilon} also harbored extensive somatic mutations. From these findings in splenic B lymphocytes, and those of a previous study of blood lymphocytes, it seems that preferential usage of V{sub H}5 genes and extensive somatic hypermutation are characteristic of B cells synthesizing IgE in patients with allergic disease. 27 refs., 3 figs., 2 tabs.
- OSTI ID:
- 83484
- Journal Information:
- Journal of Immunology, Journal Name: Journal of Immunology Journal Issue: 10 Vol. 154; ISSN JOIMA3; ISSN 0022-1767
- Country of Publication:
- United States
- Language:
- English
Similar Records
Use of the most J[sub H]-proximal human Ig H chain V region gene, V[sub H6], in the expressed immune repertoire
A majority of Ig H chain cDNA of normal human adult blood lymphocytes resembles cDNA for fetal Ig and natural autoantibodies
Myeloma Ig heavy chain V region sequences reveal prior antigenic selection and marked somatic mutation but no intraclonal diversity
Journal Article
·
Tue Jun 01 00:00:00 EDT 1993
· Journal of Immunology; (United States)
·
OSTI ID:6180400
A majority of Ig H chain cDNA of normal human adult blood lymphocytes resembles cDNA for fetal Ig and natural autoantibodies
Journal Article
·
Sun Nov 14 23:00:00 EST 1993
· Journal of Immunology; (United States)
·
OSTI ID:7076258
Myeloma Ig heavy chain V region sequences reveal prior antigenic selection and marked somatic mutation but no intraclonal diversity
Journal Article
·
Fri Sep 01 00:00:00 EDT 1995
· Journal of Immunology
·
OSTI ID:160165