Construction and analysis of Pst I DNA library for RFLP mapping of the rye genome
Journal Article
·
· Russian Journal of Genetics
OSTI ID:83446
- Institute of Genetics and Cytology, Minsk (Belarus)
- Institute of Plant Genetics and Crop Plant Research, Gatersleben (Germany)
Pst I, a methylation-sensitive restriction enzyme, was used for producing a library of rye genome DNA rich in low-copy sequences, and intended as probes for genetic mapping. Dot-hybridization and Southern blot analysis showed that 43.6% of the library is represented by low-copy DNA sequences. To locate these sequences on chromosomes and determine the degree of their repetitiveness, 11 clones were hybridized with DNA of nulli-tetrasomic lines of Chinese Spring wheat, wheat-rye addition lines, and barley cleaved by Hind III, EcoR I, EcoR V, Dra I, and BamH I restriction enzymes. Each of the rye DNA clones studied hybridized with wheat and barley DNA, suggesting that low-copy Pst I clones of rye correspond to the evolutionary conservative DNA fraction in cereals. 21 refs., 3 figs., 2 tabs.
- OSTI ID:
- 83446
- Journal Information:
- Russian Journal of Genetics, Journal Name: Russian Journal of Genetics Journal Issue: 6 Vol. 31; ISSN RJGEEQ; ISSN 1022-7954
- Country of Publication:
- United States
- Language:
- English
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