RNA structure and scalar coupling constants
- Univ. of California, Berkeley, CA (United States)
Signs and magnitudes of scalar coupling constants-spin-spin splittings-comprise a very large amount of data that can be used to establish the conformations of RNA molecules. Proton-proton and proton-phosphorus splittings have been used the most, but the availability of {sup 13}C-and {sup 15}N-labeled molecules allow many more coupling constants to be used for determining conformation. We will systematically consider the torsion angles that characterize a nucleotide unit and the coupling constants that depend on the values of these torsion angles. Karplus-type equations have been established relating many three-bond coupling constants to torsion angles. However, one- and two-bond coupling constants can also depend on conformation. Serianni and coworkers measured carbon-proton coupling constants in ribonucleosides and have calculated their values as a function of conformation. The signs of two-bond coupling can be very useful because it is easier to measure a sign than an accurate magnitude.
- Research Organization:
- Los Alamos National Lab., NM (United States)
- DOE Contract Number:
- FG03-86ER60406; FG05-86ER75281
- OSTI ID:
- 83385
- Report Number(s):
- LA--12893-C; CONF-9403228--; ON: DE95012795
- Country of Publication:
- United States
- Language:
- English
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