Monitoring Genetic and Metabolic Potential for In-Situ Bioremediation: Mass Spectrometry

Technical Report · Thu Jun 01 04:00:00 EDT 2000

DOI:https://doi.org/10.2172/827403· OSTI ID:827403

Buchanan, Michelle V; Britt, Phillip F; Doktycz, Mitchel J; Hurst, Gregory B; Lidstrom, Mary E

A number of DOE sites are contaminated with dense non-aqueous phase liquids (DNAPLs) such as carbon tetrachloride and trichloroethylene. At many of these sites, microbial bioremediation is an attractive strategy for cleanup, since it has the potential to degrade DNAPLs in situ. A rapid screening method to determine the broad range potential of a site's microbial population for contaminant degradation would greatly facilitate assessment for in situ bioremediation, as well as for monitoring ongoing bioremediation treatment. Current laboratory-based treatability methods are cumbersome and expensive. In this project, we are developing methods based on matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for rapid and accurate detection of polymerase chain reaction (PCR) products from microbial genes involved in biodegradation of pollutants. PCR primers are being developed to amplify DNA sequences that are amenable to MALDI-MS detection. This work will lay the foundation for development of a field-portable MS-based technique for rapid on site assessment and monitoring of bioremediation processes.

• Research Organization: Oak Ridge National Lab., TN; University of Washington, Seattle, WA (US)
• Sponsoring Organization: USDOE Office of Environmental Management (EM) (US)
• OSTI ID: 827403
• Report Number(s): EMSP-55108--2000
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIODEGRADATION
BIOREMEDIATION
CARBON TETRACHLORIDE
DETECTION
DNA
GENES
GENETICS
LASERS
MASS SPECTROSCOPY
MONITORING
POLLUTANTS
POLYMERASE CHAIN REACTION