Genomic structure, gene expression, and promoter analysis of human multidrug resistance-associated protein 7
The multidrug resistance-associated protein (MRP) subfamily transporters associated with anticancer drug efflux are attributed to the multidrug-resistance of cancer cells. The genomic organization of human multidrug resistance-associated protein 7 (MRP7) was identified. The human MRP7 gene, consisting of 22 exons and 21 introns, greatly differs from other members of the human MRP subfamily. A splicing variant of human MRP7, MRP7A, expressed in most human tissues, was also characterized. The 1.93-kb promoter region of MRP7 was isolated and shown to support luciferase activity at a level 4- to 5-fold greater than that of the SV40 promoter. Basal MRP7 gene expression was regulated by 2 regions in the 5-flanking region at 1,780 1,287 bp, and at 611 to 208 bp. In Madin-Darby canine kidney (MDCK) cells, MRP7 promoter activity was increased by 226 percent by genotoxic 2-acetylaminofluorene and 347 percent by the histone deacetylase inhibitor, trichostatin A. The protein was expressed in the membrane fraction of transfected MDCK cells.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE Director. Office of Science. Biological and Environmental Research; National Science Council of the Republic of China, Taiwan Grant NSC89-2320-B-006-008 (US)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 824631
- Report Number(s):
- LBNL-52927; R&D Project: LGFGAA; TRN: US200419%%165
- Journal Information:
- Journal Of Biomedical Science, Vol. 10; Other Information: Journal Publication Date: 2003; PBD: 15 Mar 2002
- Country of Publication:
- United States
- Language:
- English
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