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Nuclear apoJ: An X-ray-inducible cell death signal. Final Report

Technical Report ·
DOI:https://doi.org/10.2172/824533· OSTI ID:824533
 [1]
  1. Case Western Reserve Univ., Cleveland, OH (United States); Case Western Reserve University
The complex interactions between apoptosis, genomic instability and carcinogenesis induced by low dose ionizing radiation (IR) are poorly understood. Tissues differentially withstand IR based on complex processes that include DNA repair, altered gene expression, and apoptosis. This proposal is based on the investigators' discovery of a protein, apoJ (initially designated xip8/XIP8), that is dramatically IR-induced. The protein is also known as a marker for apoptosis, but its function is unknown. The ''nuclear'' form of the apoJ protein, undergoes dramatic accumulation in the nucleus following IR, and then strongly associates with the C-terminus of Ku70, a key factor in DNA-PK-dependent nonhomologous DNA double strand break (DSB) repair. These studies suggest that the nuclear form of apoJ is a major determinant in the elimination of carcinogenic cells and that it contributes strongly to nonlinearity threshold responses for survival and carcinogenesis.
Research Organization:
Case Western Reserve Univ., Cleveland, OH (United States)
Sponsoring Organization:
USDOE Office of Energy Research (ER)
DOE Contract Number:
FG02-99ER62724
OSTI ID:
824533
Report Number(s):
DOE/ER/--62724-1
Country of Publication:
United States
Language:
English

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