High-throughput proteomics: A flexible and efficient pipeline for protein production
Journal Article
·
· Journal of Proteome Research
- LBNL Library
Many studies that aim to characterize the proteome require the production of pure protein in a high-throughput format. We have developed a system for high-throughput subcloning, protein expression and purification that is simple, fast and inexpensive. We utilized ligation-independent cloning with a custom-designed vector and developed an expression screen to test multiple parameters for optimal protein production in E. coli. A 96-well format purification protocol was also developed that produced microgram quantities of pure protein.
- Research Organization:
- Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
- Sponsoring Organization:
- USDOE Director. Office of Science. Biological and Environmental Research (US)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 823199
- Report Number(s):
- LBNL--51265
- Journal Information:
- Journal of Proteome Research, Journal Name: Journal of Proteome Research Journal Issue: 6 Vol. 1
- Country of Publication:
- United States
- Language:
- English
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