Regulatory elements of the floral homeotic gene AGAMOUS identified by phylogenetic footprinting and shadowing.
OAK-B135 In Arabidopsis thaliana, cis-regulatory sequences of the floral homeotic gene AGAMOUS (AG) are located in the second intron. This 3 kb intron contains binding sites for two direct activators of AG, LEAFY (LFY) and WUSCHEL (WUS), along with other putative regulatory elements. We have used phylogenetic footprinting and the related technique of phylogenetic shadowing to identify putative cis-regulatory elements in this intron. Among 29 Brassicaceae, several other motifs, but not the LFY and WUS binding sites previously identified, are largely invariant. Using reporter gene analyses, we tested six of these motifs and found that they are all functionally important for activity of AG regulatory sequences in A. thaliana. Although there is little obvious sequence similarity outside the Brassicaceae, the intron from cucumber AG has at least partial activity in A. thaliana. Our studies underscore the value of the comparative approach as a tool that complements gene-by-gene promoter dissection, but also highlight that sequence-based studies alone are insufficient for a complete identification of cis-regulatory sites.
- Research Organization:
- Salk Institute (US)
- Sponsoring Organization:
- USDOE Office of Energy Research (ER) (US)
- DOE Contract Number:
- FG03-98ER20317
- OSTI ID:
- 821972
- Report Number(s):
- DOE-ER20317-3
- Journal Information:
- Plant Cell, Journal Name: Plant Cell Vol. 15; ISSN 1296-1309
- Country of Publication:
- United States
- Language:
- English
Similar Records
Progress Report for DOE DE-FG03-98ER20317 ''Regulation of the floral homeotic gene AGAMOUS'' Current and Final Funding Period: September 1, 2002, to December 31, 2002
Activation of floral homeotic genes in arabidopsis
Functional Evolution of cis-Regulatory Modules at a Homeotic Gene in Drosophila
Technical Report
·
Mon Mar 10 23:00:00 EST 2003
·
OSTI ID:821980
Activation of floral homeotic genes in arabidopsis
Journal Article
·
Fri Sep 24 00:00:00 EDT 1993
· Science (Washington, D.C.); (United States)
·
OSTI ID:5241365
Functional Evolution of cis-Regulatory Modules at a Homeotic Gene in Drosophila
Journal Article
·
Thu Nov 05 19:00:00 EST 2009
· PLoS Genetics
·
OSTI ID:1627279