Capillary electrophoresis-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for direct analysis of cellular proteins
Journal Article
·
· Analytical Chemistry (Washington)
- Pacific Northwest Lab., Richland, WA (United States)
- Pennsylvania State Univ., University Park, PA (United States)
The combination of capillary electrophoresis (CE) with electrospray ionization (ESI) mass spectrometry has proven to be broadly applicable to a wide range of biologically important compounds. When combined with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry, the combined method, in addition to high-resolution separations, affords high-resolution precision mass measurements for analytes separated from complex mixtures. Direct chemical analysis of single cells has received considerable attention in recent years; the single cell approach provides a major step toward answering important questions in the field of cellular biochemistry. In this work we present preliminary results which demonstrate the feasibility of using the CE-ESI-FTICR combination as a high-performance detection scheme for the analysis of cellular proteins acquired directly from small populations (i.e., 5-10) of intact living cells. The human erythrocyte was chosen as a model system owing to its availability, relatively homogeneous composition, and thorough documentation of contents by previous researchers. In this work we demonstrate the on-line acquisition of high-resolution mass spectra (average resolution >45 000 fwhm) of both the {alpha} and the {beta} chains of hemoglobin acquired from the injection of 10 human erythrocytes. 33 refs., 3 figs.
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 81425
- Journal Information:
- Analytical Chemistry (Washington), Journal Name: Analytical Chemistry (Washington) Journal Issue: 8 Vol. 67; ISSN ANCHAM; ISSN 0003-2700
- Country of Publication:
- United States
- Language:
- English
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