Final Report Nucleic Acid System - PCR, Multiplex Assays and Sample Preparation Project

Technical Report · Fri Apr 20 04:00:00 EDT 2001

DOI:https://doi.org/10.2172/802611· OSTI ID:802611

Koopman, R P ^[1]; Langlois, R G; Nasarabadi, S; Benett, W J; Richards, J B; Hadley, D R; Miles, R R; Brown, S B; Stratton, P L; Milanovich, F P

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1. LLNL

The objective of this project was to reduce to practice the detection and identification of biological warfare pathogens by the nucleic acid recognition technique of PCR (polymerase chain reaction). This entailed not only building operationally functional instrumentation but also developing the chemical assays for detection of priority pathogens. This project had two principal deliverables: (1) design, construct, test and deliver a 24 chamber, multiplex capable suitcase sized PCR instrument, and (2) develop and reduce to practice a multiplex assay for the detection of PCR product by flow cytometry. In addition, significant resources were allocated to test and evaluation of the Hand-held Advanced Nucleic Acid Analyzer (HANAA). This project helps provide the signature and intelligence gathering community the ability to perform, on-site or remote, rapid analysis of environmental or like samples for the presence of a suite of biological warfare pathogens.

• Research Organization: Lawrence Livermore National Lab., CA (US)
• Sponsoring Organization: USDOE Office of Defense Programs (DP) (US)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 802611
• Report Number(s): UCRL-ID-144084
• Country of Publication: United States
• Language: English

Related Subjects

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL WARFARE
CHAINS
DESIGN
DETECTION
EVALUATION
FUNCTIONALS
NUCLEIC ACIDS
PATHOGENS
SAMPLE PREPARATION