Construction and characterization of human and mouse BAC libraries from sheared DNA
Technical Report
·
OSTI ID:799295
- California Institute of Technology
We have developed a new way to construct BAC libraries with small inserts using sheared DNA sources. Because of our use of the randomly sheared DNA as DNA sources, some regions of genome may be represented better in our libraries compared to the currently available and more conventional libraries constructed by enzymatic partial digestion. B263 We have developed a new fingerprinting method useful for physical mapping by large insert clones, in particular by BACs. It is based on four-color fluorescent labeling of fragments generated by combination of a type II and a type IIS restriction enzyme.
- Research Organization:
- California Institute of Technology, Pasadena, CA (US)
- Sponsoring Organization:
- USDOE Office of Science (US)
- DOE Contract Number:
- FG03-99ER62855
- OSTI ID:
- 799295
- Country of Publication:
- United States
- Language:
- English
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