Evaluation of assays for the identification and quantitation of muconic acid, a benzene metabolite in human urine
- Univ. of Medicine and Dentistry of New Jersey, Piscataway, NJ (United States)
- Inhalation Toxicology Research Institute, Albuquerque, NM (United States)
Muconic acid (MA) is a urinary metabolite of benzene and has been used as a biomarker of exposure to benzene in humans exposed to levels as low as 1 ppm. We have modified a high-pressure liquid chromatography (HPLC) based assay for urinary MA by the use of a diode array detector. This modification increases the specificity of the HPLC-based assay by identifying false positives. In addition, we have developed a gas chromatography (GC) based assay that uses a flame ionization detector (GC-FID). Both assays identified and quantified MA in human urine at concentrations greater than 40-50 ng/ml. Assay precision was within 10% relative standard deviation for MA concentrations above 90 ng/ml using the HPLC assay and above 40 ng/ml using the GC-FID assay. Quantitative accuracy of the assays was evaluated by determining MA in human urine samples using both methods and also a gas chromatography-mass spectrometry (GC-MS) procedure. Numerical correlation among the three assays was good at MA concentrations above 100 ng/ml. 26 refs., 3 figs., 2 tabs.
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC04-76EV01013
- OSTI ID:
- 79414
- Journal Information:
- Journal of Toxicology and Environmental Health, Journal Name: Journal of Toxicology and Environmental Health Journal Issue: 3 Vol. 42; ISSN 0098-4108; ISSN JTEHD6
- Country of Publication:
- United States
- Language:
- English
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