Chromosomal damage and repair in G{sub 1}-phase Chinese hamster ovary cells exposed to charged-particle beams
- Lawrence Berkeley Lab., CA (United States)
Chromosomal fragmentation was examined in G{sub 1}-phase Chinese hamster ovary cells using the premature chromosome condensation (PCC) technique. The yield and distribution of chromatin breaks, the lesions revealed by PCC, were measured in cells exposed to X rays or each of nine particle beams covering a range of LET from 0.56 to 2700 keV/{mu}m. The average number of breaks per cell was found to be linearly proportional to the fluence of high-LET neon ions (183 keV/{mu}m). Assuming a linear response for the other beams, the level of breakage per unit dose rose from a plateau at the lowest LET values to a peak in the 100-200 keV/{mu}m range and then declined continuously thereafter, eventually falling well below the low-LET plateau. The maximum breakage RBE was 1.5. The average number of breaks per particle traversal rose steadily from 0.006 to 11 breaks/cell as the LET increased from 0.56 to 2700 keV/{mu}m. The breaks were distributed randomly within the cell population after low-LET irradiation, but became progressively overdispersed with increasing LET. Rejoining of prematurely condensed chromosomes plus fragments was followed for up to 5 h for four particle beams having LET values between 0.56 and 183 keV/{mu}m. An LET-dependent trend toward higher levels of residual fragments was observed. 34 refs., 5 figs., 4 tabs.
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 79312
- Journal Information:
- Radiation Research, Journal Name: Radiation Research Journal Issue: 3 Vol. 138; ISSN RAREAE; ISSN 0033-7587
- Country of Publication:
- United States
- Language:
- English
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