Energetics and kinetics of anaerobic aromatic and fatty acid degradation. Progress report, November 1993--November 1994
Factors influencing the rate and extent of benzoate degradation by the anaerobic syntrophic consortia were studied. Nonlinear regression analysis showed that the cause of the benzoate threshold was not a diminished benzoate degradation capacity. Analysis of cocultures with hydrogen users that differed in their hydrogen utilization capacities showed that the threshold did not depend on the kinetic properties of the syntrophic partner. These data support a thermodynamic explanation for the threshold, and exclude the possibility that a change in the affinity of the enzyme system due to acetate inhibition caused the threshold. Modeling studies showed that the threshold value could be predicted from the concentrations of the end products, assuming a critical Gibb`s free energy value. This work shows that interspecies acetate transfer is important in controlling the extent of metabolism by syntrophic organisms.
- Research Organization:
- Oklahoma Univ., Norman, OK (United States). Dept. of Botany and Microbiology
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG05-89ER14003
- OSTI ID:
- 79062
- Report Number(s):
- DOE/ER/14003--6; ON: DE95013068
- Country of Publication:
- United States
- Language:
- English
Similar Records
Energetics and kinetics of anaerobic aromatic and fatty acid degradation. Progress report, November 1992--November 1993
Energetics and kinetics of anaerobic aromatic and fatty acid degradation