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Enhanced Detection of Proteins in Microchip Separations by On-Chip Preconcentration

Conference ·
OSTI ID:788571

Microfluidic chips incorporating a semiporous glass filter were used to electrokinetically concentrate proteins on-chip prior to injection and electrophoretic analysis. Signal enhancements of >100-fold could be achieved for the microchip analysis of both native and SDS-denatured proteins using this technique.

Research Organization:
Oak Ridge National Lab., TN (US)
Sponsoring Organization:
US Department of Energy (US)
DOE Contract Number:
AC05-00OR22725
OSTI ID:
788571
Report Number(s):
P01-110929
Country of Publication:
United States
Language:
English

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