Crystallization of a complex between human CDK6 and a virus-encoded cyclin is critically dependent on the addition of small charged organic molecules
Journal Article
·
· Acta Crystallographica D
- LBNL Library
Human CDK6 plays an important role in controlling entry into the eucaryotic cell cycle. An activated complex of human CDK6 with a viral cyclin from herpesvirus saimiri was purified to homogeneity and crystallized using polyethylene glycol 3350 as precipitant. Crystallization was critically dependent on a narrow range of Ca Acetate concentration and the presence of Sulfo-betaine 201 as additive. Crystals belong to the hexagonal space group P6122 or P6522 with unit cell parameters a = b = 70.14 Angstrom, c = 448.77 Angstrom, g = 120 degrees and diffract X-rays to at least 3.1 Angstrom resolution.
- Research Organization:
- Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
- Sponsoring Organization:
- USDOE Director, Office of Science. Office of Biological and Environmental Research; National Institutes of Health (US)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 786985
- Report Number(s):
- LBNL--47849
- Journal Information:
- Acta Crystallographica D, Journal Name: Acta Crystallographica D Journal Issue: PT9 Vol. 57
- Country of Publication:
- United States
- Language:
- English
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