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Title: Microarray expression profiling identifies genes with altered expression in HDL-deficient mice

Abstract

Based on the assumption that severe alterations in the expression of genes known to be involved in HDL metabolism may affect the expression of other genes we screened an array of over 5000 mouse expressed sequence tags (ESTs) for altered gene expression in the livers of two lines of mice with dramatic decreases in HDL plasma concentrations. Labeled cDNA from livers of apolipoprotein AI (apo AI) knockout mice, Scavenger Receptor BI (SR-BI) transgenic mice and control mice were co-hybridized to microarrays. Two-sample t-statistics were used to identify genes with altered expression levels in the knockout or transgenic mice compared with the control mice. In the SR-BI group we found 9 array elements representing at least 5 genes to be significantly altered on the basis of an adjusted p value of less than 0.05. In the apo AI knockout group 8 array elements representing 4 genes were altered compared with the control group (p < 0.05). Several of the genes identified in the SR-BI transgenic suggest altered sterol metabolism and oxidative processes. These studies illustrate the use of multiple-testing methods for the identification of genes with altered expression in replicated microarray experiments of apo AI knockout and SR-BI transgenic mice.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab., CA (US)
Sponsoring Org.:
USDOE Director, Office of Science. Office of Biological and Environmental Research. Life Sciences Division; National Institutes of Health (US)
OSTI Identifier:
775124
Report Number(s):
LBNL-45746
R&D Project: 860K11; TRN: AH200110%%111
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Journal Article
Journal Name:
Genome Research
Additional Journal Information:
Journal Volume: 10; Journal Issue: 12; Other Information: Journal Publication Date:December 2000; PBD: 5 May 2000
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; LIVER; APOLIPOPROTEINS; GENES; METABOLISM; MICE; STEROLS; TRANSGENIC MICE; MICROARRAY APOLIPOPROTEIN SR-BI RECEPTOR HDL

Citation Formats

Callow, Matthew J., Dudoit, Sandrine, Gong, Elaine L., Speed, Terence P., and Rubin, Edward M. Microarray expression profiling identifies genes with altered expression in HDL-deficient mice. United States: N. p., 2000. Web. doi:10.1101/gr.10.12.2022.
Callow, Matthew J., Dudoit, Sandrine, Gong, Elaine L., Speed, Terence P., & Rubin, Edward M. Microarray expression profiling identifies genes with altered expression in HDL-deficient mice. United States. doi:10.1101/gr.10.12.2022.
Callow, Matthew J., Dudoit, Sandrine, Gong, Elaine L., Speed, Terence P., and Rubin, Edward M. Fri . "Microarray expression profiling identifies genes with altered expression in HDL-deficient mice". United States. doi:10.1101/gr.10.12.2022.
@article{osti_775124,
title = {Microarray expression profiling identifies genes with altered expression in HDL-deficient mice},
author = {Callow, Matthew J. and Dudoit, Sandrine and Gong, Elaine L. and Speed, Terence P. and Rubin, Edward M.},
abstractNote = {Based on the assumption that severe alterations in the expression of genes known to be involved in HDL metabolism may affect the expression of other genes we screened an array of over 5000 mouse expressed sequence tags (ESTs) for altered gene expression in the livers of two lines of mice with dramatic decreases in HDL plasma concentrations. Labeled cDNA from livers of apolipoprotein AI (apo AI) knockout mice, Scavenger Receptor BI (SR-BI) transgenic mice and control mice were co-hybridized to microarrays. Two-sample t-statistics were used to identify genes with altered expression levels in the knockout or transgenic mice compared with the control mice. In the SR-BI group we found 9 array elements representing at least 5 genes to be significantly altered on the basis of an adjusted p value of less than 0.05. In the apo AI knockout group 8 array elements representing 4 genes were altered compared with the control group (p < 0.05). Several of the genes identified in the SR-BI transgenic suggest altered sterol metabolism and oxidative processes. These studies illustrate the use of multiple-testing methods for the identification of genes with altered expression in replicated microarray experiments of apo AI knockout and SR-BI transgenic mice.},
doi = {10.1101/gr.10.12.2022},
journal = {Genome Research},
number = 12,
volume = 10,
place = {United States},
year = {2000},
month = {5}
}