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Title: Library Resources for Bac End Sequencing. Final Technical Report

Abstract

Studies directed towards the specific aims outlined for this research award are summarized. The RPCI II Human Bac Library has been expanded by the addition of 6.9-fold genomic coverage. This segment has been generated from a MBOI partial digest of the same anonymous donor DNA used for the rest of the library. A new cloning vector, pTARBAC1, has been constructed and used in the construction of RPCI-II segment 5. This new cloning vector provides a new strategy in identifying targeted genomic regions and will greatly facilitate a large-scale analysis for positional cloning. A new maleCS7BC/6J mouse BAC library has been constructed. RPCI-23 contain 576 plates (approx 210,000 clones) and represents approximately 11-fold coverage of the mouse genome.

Authors:
Publication Date:
Research Org.:
Health Research Inc., Buffalo, NY (US)
Sponsoring Org.:
US Department of Energy (US)
OSTI Identifier:
772661
DOE Contract Number:  
FG02-98ER62675
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 1 Oct 2000
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AWARDS; CLONING; CONSTRUCTION; DNA; PLATES; VECTORS

Citation Formats

Pieter J. de Jong. Library Resources for Bac End Sequencing. Final Technical Report. United States: N. p., 2000. Web. doi:10.2172/772661.
Pieter J. de Jong. Library Resources for Bac End Sequencing. Final Technical Report. United States. doi:10.2172/772661.
Pieter J. de Jong. Sun . "Library Resources for Bac End Sequencing. Final Technical Report". United States. doi:10.2172/772661. https://www.osti.gov/servlets/purl/772661.
@article{osti_772661,
title = {Library Resources for Bac End Sequencing. Final Technical Report},
author = {Pieter J. de Jong},
abstractNote = {Studies directed towards the specific aims outlined for this research award are summarized. The RPCI II Human Bac Library has been expanded by the addition of 6.9-fold genomic coverage. This segment has been generated from a MBOI partial digest of the same anonymous donor DNA used for the rest of the library. A new cloning vector, pTARBAC1, has been constructed and used in the construction of RPCI-II segment 5. This new cloning vector provides a new strategy in identifying targeted genomic regions and will greatly facilitate a large-scale analysis for positional cloning. A new maleCS7BC/6J mouse BAC library has been constructed. RPCI-23 contain 576 plates (approx 210,000 clones) and represents approximately 11-fold coverage of the mouse genome.},
doi = {10.2172/772661},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Oct 01 00:00:00 EDT 2000},
month = {Sun Oct 01 00:00:00 EDT 2000}
}

Technical Report:

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