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Title: Towards Chip Scale Liquid Chromatography and High Throughput Immunosensing

Abstract

This work describes several research projects aimed towards developing new instruments and novel methods for high throughput chemical and biological analysis. Approaches are taken in two directions. The first direction takes advantage of well-established semiconductor fabrication techniques and applies them to miniaturize instruments that are workhorses in analytical laboratories. Specifically, the first part of this work focused on the development of micropumps and microvalves for controlled fluid delivery. The mechanism of these micropumps and microvalves relies on the electrochemically-induced surface tension change at a mercury/electrolyte interface. A miniaturized flow injection analysis device was integrated and flow injection analyses were demonstrated. In the second part of this work, microfluidic chips were also designed, fabricated, and tested. Separations of two fluorescent dyes were demonstrated in microfabricated channels, based on an open-tubular liquid chromatography (OT LC) or an electrochemically-modulated liquid chromatography (EMLC) format. A reduction in instrument size can potentially increase analysis speed, and allow exceedingly small amounts of sample to be analyzed under diverse separation conditions. The second direction explores the surface enhanced Raman spectroscopy (SERS) as a signal transduction method for immunoassay analysis. It takes advantage of the improved detection sensitivity as a result of surface enhancement on colloidal gold, themore » narrow width of Raman band, and the stability of Raman scattering signals to distinguish several different species simultaneously without exploiting spatially-separated addresses on a biochip. By labeling gold nanoparticles with different Raman reporters in conjunction with different detection antibodies, a simultaneous detection of a dual-analyte immunoassay was demonstrated. Using this scheme for quantitative analysis was also studied and preliminary dose-response curves from an immunoassay of a mo del antigen were obtained. Simultaneous detection of several analytes at the same address can potentially increase the analysis speed, and can further expand the analysis capability of a microarray chip.« less

Authors:
 [1]
  1. Iowa State Univ., Ames, IA (United States)
Publication Date:
Research Org.:
Ames Lab., Ames, IA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
764621
Report Number(s):
IS-T 1917
TRN: AH200103%%591
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: TH: Thesis; PBD: 21 Sep 2000
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; ANTIBODIES; ANTIGENS; CHROMATOGRAPHY; DETECTION; FABRICATION; GOLD; IMMUNOASSAY; RAMAN SPECTROSCOPY; SCATTERING; SURFACE TENSION

Citation Formats

Ni, Jing. Towards Chip Scale Liquid Chromatography and High Throughput Immunosensing. United States: N. p., 2000. Web. doi:10.2172/764621.
Ni, Jing. Towards Chip Scale Liquid Chromatography and High Throughput Immunosensing. United States. https://doi.org/10.2172/764621
Ni, Jing. Thu . "Towards Chip Scale Liquid Chromatography and High Throughput Immunosensing". United States. https://doi.org/10.2172/764621. https://www.osti.gov/servlets/purl/764621.
@article{osti_764621,
title = {Towards Chip Scale Liquid Chromatography and High Throughput Immunosensing},
author = {Ni, Jing},
abstractNote = {This work describes several research projects aimed towards developing new instruments and novel methods for high throughput chemical and biological analysis. Approaches are taken in two directions. The first direction takes advantage of well-established semiconductor fabrication techniques and applies them to miniaturize instruments that are workhorses in analytical laboratories. Specifically, the first part of this work focused on the development of micropumps and microvalves for controlled fluid delivery. The mechanism of these micropumps and microvalves relies on the electrochemically-induced surface tension change at a mercury/electrolyte interface. A miniaturized flow injection analysis device was integrated and flow injection analyses were demonstrated. In the second part of this work, microfluidic chips were also designed, fabricated, and tested. Separations of two fluorescent dyes were demonstrated in microfabricated channels, based on an open-tubular liquid chromatography (OT LC) or an electrochemically-modulated liquid chromatography (EMLC) format. A reduction in instrument size can potentially increase analysis speed, and allow exceedingly small amounts of sample to be analyzed under diverse separation conditions. The second direction explores the surface enhanced Raman spectroscopy (SERS) as a signal transduction method for immunoassay analysis. It takes advantage of the improved detection sensitivity as a result of surface enhancement on colloidal gold, the narrow width of Raman band, and the stability of Raman scattering signals to distinguish several different species simultaneously without exploiting spatially-separated addresses on a biochip. By labeling gold nanoparticles with different Raman reporters in conjunction with different detection antibodies, a simultaneous detection of a dual-analyte immunoassay was demonstrated. Using this scheme for quantitative analysis was also studied and preliminary dose-response curves from an immunoassay of a mo del antigen were obtained. Simultaneous detection of several analytes at the same address can potentially increase the analysis speed, and can further expand the analysis capability of a microarray chip.},
doi = {10.2172/764621},
url = {https://www.osti.gov/biblio/764621}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {9}
}

Thesis/Dissertation:
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