Enumeration of petroleum-degrading microorganisms
A variety of factors, including concentration of oil, antibiotics, dyes, and inoculum washes, were examined to determine their effect on the total counts of microorganisms on oil-containing media. The media found to be best for enumerating petroleum-degrading microorganisms contained 0.5 percent (vol/vol) oil and 0.003 percent phenol red, with Fungizone added for isolating bacteria and streptomycin and tetracycline added for isolating yeasts and fungi. Washing the inoculum did not improve recovery of petroleum degraders. Specifically, silica gel-oil medium and a yeast medium are recommended for enumeration of petroleum-degrading bacteria and yeasts and fungi, respectively. It is suggested that counts of petroleum degraders be expressed as percentage of the total population rather than total numbers of petroleum degraders per se. Incubation temperature and presence of oil was found to influence the numbers of petroleum-degrading microorganisms at a given sampling site. (auth)
- Research Organization:
- Univ. of Maryland, College Park
- OSTI ID:
- 7365823
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 31:2; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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550700 -- Microbiology
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADSORBENTS
ANTIBIOTICS
AQUATIC ECOSYSTEMS
BACTERIA
BIOASSAY
BIODEGRADATION
BIOMASS
CHEMICAL REACTIONS
COUNTING TECHNIQUES
DECOMPOSITION
DRUGS
ECOSYSTEMS
ENERGY SOURCES
ENVIRONMENTAL EFFECTS
FOSSIL FUELS
FUELS
FUNGI
MICROORGANISMS
OILS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
PETROLEUM
PLANTS
POLLUTION
POPULATION DENSITY
QUANTITY RATIO
RENEWABLE ENERGY SOURCES
SEPARATION PROCESSES
SILICA GEL
STREPTOMYCIN
TETRACYCLINES
WATER POLLUTION
YEASTS