Strand breaks, base release and post-irradiation changes in DNA. gamma. -irradiated in dilute O/sub 2/-saturated aqueous solution
Gamma irradiation of DNA in dilute O/sub 2/-saturated aqueous solution releases free bases and damaged bases from the macromolecule. The yields of these products were measured after column chromatographic separation. For double stranded DNA the immediate yield of bases varies from G = 0.012 for cytosine to G = 0.033 for adenine. The yields of released bases increase with post-irradiation time (the majority of the increase occurs in the first 2 hrs.) to yields in the range of G = 0.07 +- 0.012. Yields of two released damaged thymine radiation products from ..gamma..-irradiated /sup 3/H thymine labelled DNA also increased with post-irradiation time. Strand breaks were measured in ..gamma..-irradiated single stranded DNA the initial yield G = 0.02 was low but increased with time to G = 0.07. No direct correlation between strand-break production and release of low molecular weight products is possible. The findings are discussed in terms of damage to DNA in vivo and its enzymatic repair.
- Research Organization:
- California Univ., Los Angeles (USA). Lab. of Nuclear Medicine and Radiation Biology
- DOE Contract Number:
- E(04-1)-GEN-12
- OSTI ID:
- 7363227
- Report Number(s):
- UCLA-12-1060; TRN: 76-016483
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL REPAIR
STRAND BREAKS
RADIOINDUCTION
ADENINES
CYTOSINE
GAMMA RADIATION
THYMINE
AMINES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
URACILS
560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)