Antigenic analysis of the major structural protein of the Mason-Pfizer monkey virus. [/sup 125/I tracer technique]

Journal Article · Thu Jul 01 00:00:00 EDT 1976 · J. Immunol.; (United States)

OSTI ID:7362358

Schochetman, G; Boehm-Truitt, M; Schlom, J

The major internal protein, p27 (m.w. 27,000 daltons) of the Mason-Pfizer monkey virus (MPMV) was purified by gel filtration and ion-exchange chromatography and then used to develop a radioimmunoassay (RIA). This RIA was specific for MPMV because no immunologic cross-reactivity was observed between p27 of MPMV and 13 different RNA tumor viruses of mammalian and avian origin. However, the p27 of MPMV grown in three different primate cells exhibited identical antigenic cross-reactivity. In addition, significant levels of p27 were found only in MPMV-infected cells. These results indicate that synthesis of p27 is induced after virus infection and that p27 represents a viral-coded protein.

• Research Organization: Meloy Labs., Inc., Springfield, VA
• OSTI ID: 7362358
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 117:1; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BIOSYNTHESIS
CHEMICAL ANALYSIS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VIRUSES