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Scanning electron microscopy of homing and recirculating lymphocyte populations. [X Radiation]

Journal Article · · Cell. Immunol.; (United States)
The surface structure of T and B lymphocytes in vivo was investigated using scanning electron microscopy. For these studies the spleen and mesenteric lymph node of mice enriched for B lymphocytes (adult thymectomized, lethally irradiated, bone marrow reconstituted mice, B mice) and of mice enriched for T lymphocytes (adult, lethally irradiated, thymocyte transferred mice, T mice) were examined. Both types of lymphocytes demonstrated a smooth cell surface when they were situated in their respective microenvironment, whereas recirculating T and B cells exhibited numerous microvilli on the cell surface. In postcapillary venules, known to be the major sites of entry of lymphocytes in lymph nodes, lymphocytes were in contact with the endothelial wall by means of these microvilli. While passing the endothelial lining, lymphocytes withdrew their microvilli and appeared smooth upon arrival in the lymphatic stroma. It is suggested that microvilli on the surface of lymphocytes play a role in cellular recognition mechanisms.
Research Organization:
Erasmus Univ., Rotterdam
OSTI ID:
7362334
Journal Information:
Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 19:2; ISSN CLIMB
Country of Publication:
United States
Language:
English