Elution electrophoresis as a clinical tool

Technical Report · Wed Mar 24 04:00:00 EST 1976

OSTI ID:7345597

Wilkes, G R; Bertolami, R J; Choi, W M; Terpandjian, P D

An experimental investigation of an elution electrophoresis system with a particulate gel packed column was performed. The operating parameters of the apparatus were determined with elution flow studies and electrophoresis migration studies. Flow study results indicated that sample dispersion is dependent on packing type, packing size, elution flow rate, and outlet geometry. Migration studies revealed that sample migration is dependent upon the packing type, packing size, buffer solution, and applied voltage. The best available operating system was selected from a composite of the results. Minimum criteria for feasibility were an established difference in migration rates for different pure samples and maintenance of the separation during elution. Electrophoresis at 1000 v in a 40-cm column packed with Bio-Gel P2, 200-400-mesh packing was performed on a sample for 30 min. The sample was eluted from the column at an eluent flow rate of 8 ml/hr. Measurable migration rates were obtained for an albumin sample. Migration for pure samples of A, AC, and F hemoglobins was not apparent. Also, electrophoresis of a mixture of these hemoglobins at the operating conditions optimum for albumin migration produced no separation of the hemoglobin mixture. Lack of migration and lack of separation with the hemoglobin samples led to the conclusion that the present operating conditions and apparatus are unsuitable for analysis of hemoglobins. A new column design for future experimentation is suggested.

• Research Organization: Massachusetts Inst. of Tech., Oak Ridge, Tenn. (USA). School of Chemical Engineering Practice
• OSTI ID: 7345597
• Report Number(s): ORNL/MIT-230
• Country of Publication: United States
• Language: English

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