Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Two color fluorescence sensor (26 Sep 1973) (Engineering Materials). [10 drawings; this package replaces part of CAPE-2372]

Miscellaneous ·
OSTI ID:7340668

A new flow system for quantitative analysis and sorting of small particles, particularly biological cells, was developed. Multiparameter analysis is made of fluorescent stained cells as they flow in a liquid suspension through a unique flow chamber. Electrical and electro-optical sensors measure cell volume, single or two-color fluorescence, and light scatter to determine cell discrimination. A laser beam is used to excite cell fluorescence and light scatter. Sorting is accomplished by breaking up the emerging stream into uniform droplets and sorting those conforming to preselected standards as determined by the processed signals. The drawings are given of the two-color fluorescence sensor unit. A detailed description of this system was published in the September 1973 issue of ''Review of Scientific Instruments.''

Research Organization:
Los Alamos Scientific Lab., N.Mex. (USA)
OSTI ID:
7340668
Report Number(s):
CAPE-2518
Country of Publication:
United States
Language:
English