Image analysis, flow fluorometry, and flow sorting of mammalian chromosomes
Conference
·
OSTI ID:7339231
The measurement and manipulation of chromosomes using two very different techniques are discussed. The first is absorption cytophotometry coupled to image analysis, a method which measures relative DNA content and centromere index of preidentified chromosomes within the well flattened metaphase cell on a microscope slide. The second is flow fluorometry and sorting, a technique which requires chromosomes in suspension, measures relative DNA content and sorts or separates chromosomes within specified ranges of DNA content. The image-analytic approach uses traditional preparations and can be directly coupled to traditional analysis; as presently practiced it is slow but immediately applicable to human cytogenetics. The flow fluorometric approach uses nonconventional preparations and a nonconventional chromosomal rather than cellular framework for the analysis; it is extremely rapid, unique in its ability to purify chromosomes, and potentially but not immediately applicable to clinical cytogenetics.
- Research Organization:
- California Univ., Livermore (USA). Lawrence Livermore Lab.
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 7339231
- Report Number(s):
- UCRL-78397; CONF-751219-2
- Country of Publication:
- United States
- Language:
- English
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