Properties of uvrE mutants of Escherichia coli K12. Part 1. Effects of uv irradiation on DNA metabolism
Escherichia coli K12 uvrE is a mutator strain which is highly sensitive to ultraviolet radiation. In an attempt to determine the underlying molecular basis for the UV sensitivity, a mutant and an isogenic wild type strain were compared with regard to several metabolic responses to 254 nm radiation. The introduction of single strand breaks into intracellular DNA after irradiation is normal; however, the rate of excision of pyrimidine dimers as well as of DNA degradation and final rejoining of the strand breaks is lower in the mutant as compared to the repair proficient strain. These data suggest that the uvrE gene product may be involved in a reaction between the incision and excision steps in the excision repair process. (Author) (GRA)
- Research Organization:
- Rijksverdedigingsorganisatie TNO, Rijswijk (Netherlands). Medisch Biologisch Lab. TNO
- OSTI ID:
- 7339182
- Report Number(s):
- N-75-24332; MBL-1974-25; TDCK-65293
- Resource Relation:
- Other Information: Sponsored partly by Med. Res. Council of Can
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
STRAND BREAKS
ESCHERICHIA COLI
RADIOSENSITIVITY
BIOLOGICAL REPAIR
MUTATIONS
ULTRAVIOLET RADIATION
BACTERIA
BIOLOGICAL RECOVERY
ELECTROMAGNETIC RADIATION
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RECOVERY
REPAIR
560112* - Radiation Effects on Biochemicals- In Microorganisms- (-1987)
560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987)