Evidence for the involvement of cytochrome P-450 in reduction of benzo(a)pyrene 4,5-oxide by rat liver microsomes

Journal Article · Wed Dec 31 23:00:00 EST 1975 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(76)90646-X· OSTI ID:7328017

Kato, R; Iwasaki, K; Shiraga, T; Noguchi, H

Benzo(a)pyrene 4,5-oxide is reduced to benzo(a)pyrene by microsomes in the presence of NADPH. Carbon monoxide and oxygen inhibit this reduction. The liver has highest activity which is almost lacking in new-born rats. Phenobarbital as well as 3-methylcholanthrene pretreatment increases the epoxide reduction. Additions of FMN or methylviologen stimulate the epoxide reduction; dimethylaniline N-oxide and cumene hydroperoxide are inhibitory. These results indicate that benzo(a)pyrene 4,5-oxide is reduced by the reduced form of cytochrome P-450.

• Research Organization: Fujisawa Research Labs., Osaka
• OSTI ID: 7328017
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 70:3; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
AZINES
BARBITURATES
BENZOPYRENE
BIOLOGICAL EFFECTS
BODY
CARBON COMPOUNDS
CARBON MONOXIDE
CARBON OXIDES
CELL CONSTITUENTS
CHALCOGENIDES
CHEMICAL REACTIONS
CONDENSED AROMATICS
CRYOGENIC FLUIDS
CYTOCHROMES
DIGESTIVE SYSTEM
DRUGS
ELEMENTS
EPOXIDES
FLUIDS
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYPNOTICS AND SEDATIVES
INHIBITION
LIVER
MAMMALS
MICROSOMES
NONMETALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDES
OXYGEN
OXYGEN COMPOUNDS
PHENOBARBITAL
PIGMENTS
PYRIMIDINES
RATS
REDUCTION
RODENTS
STIMULATION
VERTEBRATES