Evidence for the involvement of cytochrome P-450 in reduction of benzo(a)pyrene 4,5-oxide by rat liver microsomes

Journal Article · Thu Jan 01 00:00:00 EST 1976 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(76)90646-X· OSTI ID:7328017

Kato, R; Iwasaki, K; Shiraga, T; Noguchi, H

Benzo(a)pyrene 4,5-oxide is reduced to benzo(a)pyrene by microsomes in the presence of NADPH. Carbon monoxide and oxygen inhibit this reduction. The liver has highest activity which is almost lacking in new-born rats. Phenobarbital as well as 3-methylcholanthrene pretreatment increases the epoxide reduction. Additions of FMN or methylviologen stimulate the epoxide reduction; dimethylaniline N-oxide and cumene hydroperoxide are inhibitory. These results indicate that benzo(a)pyrene 4,5-oxide is reduced by the reduced form of cytochrome P-450.

• Research Organization: Fujisawa Research Labs., Osaka
• OSTI ID: 7328017
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 70:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBON MONOXIDE
BIOLOGICAL EFFECTS
EPOXIDES
REDUCTION
BENZOPYRENE
CYTOCHROMES
INHIBITION
LIVER
MICROSOMES
ORGANIC COMPOUNDS
OXYGEN
PHENOBARBITAL
RATS
STIMULATION
ANIMALS
AROMATICS
AZINES
BARBITURATES
BODY
CARBON COMPOUNDS
CARBON OXIDES
CELL CONSTITUENTS
CHALCOGENIDES
CHEMICAL REACTIONS
CONDENSED AROMATICS
CRYOGENIC FLUIDS
DIGESTIVE SYSTEM
DRUGS
ELEMENTS
FLUIDS
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYPNOTICS AND SEDATIVES
MAMMALS
NONMETALS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PIGMENTS
PYRIMIDINES
RODENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology