Role of apolipoprotein A-I in the structure of human serum high density lipoproteins: reconstitution studies

Journal Article · Fri Feb 25 04:00:00 EST 1977 · J. Biol. Chem.; (United States)

OSTI ID:7327986

Ritter, M C; Scanu, A M

For a better definition of the role of human serum apolipoprotein A-I (apo A-I) in high density lipoprotein structure, a systematic investigation was carried out on factors influencing the in vitro association of this apoprotein with lipids obtained from the parent high density lipoprotein (HDL); these lipids include phospholipids, free cholesterol, cholesteryl esters, and triglycerides. The results indicate that in vitro complexation of apo A-I with lipids is under kinetic control; apo A-I can generate a lipid protein complex with properties similar to those of the parent lipoprotein; the process requires well defined experimental conditions and, most importantly, the presence in solution of monomers and dimers of apo A-I; the number of apo A-I molecules incorporated into R-HDL determines the size and structure of the reassembled particle. All of these observations strongly support the essential role of apo A-I in the structure of human HDL.

• Research Organization: Univ. of Chicago
• OSTI ID: 7327986
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 252:4; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BLOOD SERUM
CHOLESTEROL
ESTERS
HYDROXY COMPOUNDS
KINETICS
LIPIDS
LIPOPROTEINS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PROTEINS
REACTION KINETICS
STEROIDS
STEROLS
TRIGLYCERIDES