Application of the indirect enzyme-labeled antibody microtest to the detection and surveillance of animal diseases. [Brucellosis, cholera, and trichinosis in cattle and swine]
Conference
·
OSTI ID:7324063
The rapid, indirect enzyme-labeled antibody (ELA) microplate test has been developed as a diagnostic and surveillance tool to aid in the control of animal disease. Data are presented, which illustrate the application of the test to viral (hog cholera), parasitic (trichinosis), and bacterial (brucellosis) diseases of animals. A greater than 95 percent correlation was observed between the hog cholera ELA test and the hog cholera serum neutralization test performed on over 2000 mixed hog cholera positive and negative field samples obtained during the 1976 New Jersey epizootic. Of 56 swine naturally infected with Trichinella spiralis at a level considered dangerous to man, all were ELA positive, while only one of 360 T. spiralis negative packing house sera was ELA positive. Preliminary experiments with bovine brucellosis (Brucella abortus) indicate that the ELA test is more sensitive than other test methods currently in use. ELA procedures should soon become tests of choice for the detection of antibodies to animal disease agents.
- Research Organization:
- Los Alamos Scientific Lab., NM (USA)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 7324063
- Report Number(s):
- LA-UR-76-1983; CONF-760955-1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Serologic test development. Progress report, July 1, 1975--June 30, 1976. [Packinghouse tests for cholera and trichinosis in swine]
Prospects for use of the ELA test in control of trichinellosis in swine
Serologic test development. Progress report, July 1, 1974--June 30, 1975. [Radioimmunoassay method for diagnosis of swine and bovine diseases]
Technical Report
·
Tue Nov 30 23:00:00 EST 1976
·
OSTI ID:7236770
Prospects for use of the ELA test in control of trichinellosis in swine
Conference
·
Wed Dec 31 23:00:00 EST 1975
·
OSTI ID:7324061
Serologic test development. Progress report, July 1, 1974--June 30, 1975. [Radioimmunoassay method for diagnosis of swine and bovine diseases]
Technical Report
·
Tue Dec 31 23:00:00 EST 1974
·
OSTI ID:7196839